Annals of clinical biochemistry.
Publisher:
Association of Clinical Biochemists.. London : Sage (2013)
Frequency: Bimonthly
Country: England
Language: English
Author(s):
Association of Clinical Biochemists.
Start Year:1969 -
ISSN:
0004-5632 (Print)
1758-1001 (Electronic)
0004-5632 (Linking)
1758-1001 (Electronic)
0004-5632 (Linking)
Impact Factor
2.2
2022
| NLM ID: | 0324055 |
| (DNLM): | A34770000(s) |
| (OCoLC): | 01481376 |
| Coden: | ACBOBU |
| LCCN: | sc 84002065 |
| Classification: | W1 AN57N |
Synthetic peptide-based electrochemiluminescence immunoassay for anti-Borna disease virus p40 and p24 antibodies in rat and horse serum. Borna disease virus (BDV) is a neurotropic pathogen that infects a wide variety of vertebrates. We have developed a new electrochemiluminescence immunoassay (ECLIA) for the detection of antibodies to BDV, using three synthetic peptides corresponding to the amino acid residues 3-20 and 338-358 of p40 and 59-79 of p24 peptide of BDV. Using the ECLIA, we examined serum samples for the presence of anti-BDV antibodies in 20 rats (experimentally BDV-infected and uninfected) and 38 horses (13 US horses, experimentally infected and uninfected, and 25 Japanese horses, feral and domestic). The ECLIA, pe...
Methods of assigning accurate values to reference serum. Part 2. The use of definitive methods, reference laboratories, transferred values and consensus values. Eight analytes (Ca, Cl, cholesterol, glucose, Mg, K, Na and urate) have been determined in one horse serum masterlot by up to six different procedures: (i) by so-called definitive methods; (ii) by a group of reference laboratories using a variety of analytical methods; (iii) using the results of two independent external quality assessment schemes; (iv) by transferring values from a human serum standard reference material analysed by definitive methods; (v) by similar transfer of values from several batches of horse reference serum previously analysed by definitive methods; and (vi) as in (v) b...