Biotechnology progress.
Publisher:
American Institute of Chemical Engineers,. Hoboken, NJ : Wiley-Blackwell (2010)
Frequency: Bimonthly, 1990-
Country: United States
Language: English
Author(s):
American Institute of Chemical Engineers., Society for Biological Engineering, American Institute of Chemical Engineers. Food, Pharmaceutical, and Bioengineering Division., American Chemical Society.
Start Year:1985 -
ISSN:
8756-7938 (Print)
1520-6033 (Electronic)
1520-6033 (Linking)
1520-6033 (Electronic)
1520-6033 (Linking)
Impact Factor
2.9
2023
| NLM ID: | 8506292 |
| (DNLM): | SR0052255(s) |
| (OCoLC): | 11666713 |
| Coden: | BIPRET |
| Classification: | W1 BI918V |
Purification of equine chorionic gonadotropin (eCG) using magnetic ion exchange adsorbents in combination with high-gradient magnetic separation. Current purification of the glycoprotein equine chorionic gonadotropin (eCG) from horse serum includes consecutive precipitation steps beginning with metaphosphoric acid pH fractionation, two ethanol precipitation steps, and dialysis followed by a numerous of fixed-bed chromatography steps up to the specific activity required. A promising procedure for a more economic purification procedure represents a simplified precipitation process requiring only onethird of the solvent, followed by the usage of magnetic ion exchange adsorbents employed together with a newly designed 'rotor-stator' type Hi...
Expression of Vitreoscilla hemoglobin is superior to horse heart myoglobin or yeast flavohemoglobin expression for enhancing Escherichia coli growth in a microaerobic bioreactor. Expression of a gene encoding hemoglobin (VHb) from the aerobic bacterium Vitreoscilla sp. in several organisms, including Escherichia coli, has been shown to improve microaerobic cell growth and enhance oxygen-dependent product formation. The suitability of VHb to enhance microaerobic metabolism has been suggested to depend on its unusual oxygen binding characteristics. To examine whether hemoproteins of other origins can also elicit the positive effects VHb exerts in microaerobic E. coli cells, we subcloned the genes encoding Vitreoscilla VHb, horse heart myoglobin (HMb), and yeast flavohemo...