Clinical chemistry.
Publisher:
P.B. Hoeber,. Oxford : Oxford University Press (2020)
Frequency: Twelve no. a year,
Country: England
Language: English
Author(s):
American Association of Clinical Chemists., American Association for Clinical Chemistry.
Start Year:1955 -
ISSN:
0009-9147 (Print)
1530-8561 (Electronic)
0009-9147 (Linking)
1530-8561 (Electronic)
0009-9147 (Linking)
Impact Factor
9.3
| NLM ID: | 9421549 |
| (DNLM): | C24980000(s) |
| (OCoLC): | 01554929 |
| Coden: | CLCHAU |
| LCCN: | 58002529 |
| Classification: | W1 CL685 |
Enzyme immunoassay for measuring 25-hydroxyvitamin D3 in serum. We developed a rapid, competitive enzyme immunoassay (EIA) for measuring 25-hydroxyvitamin D3 [25(OH)D3] in serum. The EIA was based upon 25(OH)D3-3-hemisuccinate covalently coupled to secondary amino groups grafted onto the polystyrene surface of microtiter wells. Optimal coupling conditions were established, and we found that inclusion of 40 mumol/L chloramine T, an agent not previously described for use in coupling to these plates, resulted in both more reproducible coupling as well as more than a twofold increase in the coupling efficiency. Before EIA, 25(OH)D3 was extracted from the serum...
On-line direct liquid introduction interface for micro-liquid chromatography/mass spectrometry: application to drug analysis. We describe an integrated micro-liquid chromatograph/mass spectrometer (micro-LC/MS) system capable of performing routine determinations for 1--10 ng of drugs and their metabolites extracted from biological fluids. The micro-LC is constructed from conventional "high-performance" liquid-chromatographic instrumentation by using commercially available components. The mass spectrometer is operated in the chemical ionization mode. The direct liquid introduction micro-LC/MS interface can be constructed from commercially available materials. Chromatographic and mass spectral results demonstrate the a...
Identification of alpha1-lipoproteins in crossed immunoelectrophoresis. Evans Blue dye binds selectively, but with different avidities, to five major antigens in human serum. The anodic mobility of the antigen-dye complexes is greater than that of the antigens alone in crossed immunoelectrophoresis, which is of practical value for identification. We used this characteristic to show that in some human sera there is a population of alpha1-lipoprotein molecules that migrates electrophoretically in the beta-lipoprotein region, where in conventional zone electrophoresis it could be mistaken for beta-lipoprotein. We also demonstrate that horses, unlike rabbits, rarely m...