Croatian medical journal.
Publisher:
University of Zagreb Medical School,. Zagreb : Medicinska Naklada (2001)
Frequency: Bimonthly, 2001-
Country: Croatia
Language: English
Author(s):
Sveučilište u Zagrebu. Medicinski fakultet., World Association of Croatian Physicians.
Start Year:1992 -
ISSN:
0353-9504 (Print)
1332-8166 (Electronic)
0353-9504 (Linking)
1332-8166 (Electronic)
0353-9504 (Linking)
Impact Factor
1.9
2022
| NLM ID: | 9424324 |
| (DNLM): | SR0072900(s) |
| (OCoLC): | 32566067 |
| Coden: | CMEJEN |
| LCCN: | sn 98040938 |
| Classification: | W1 ZA359P |
Genotyping horse epithelial cells from fecal matter by isolation of polymerase chain reaction products. To show that application of the polymerase chain reaction (PCR) method modified for amplification of a low-copy number DNA samples, ie, the isolation of PCR products (IPCRp), would represent improvement in obtaining genotypes from a fecal DNA compared with previously used genotyping methods. Methods: The DNA from the horse fecal matter was extracted by modified Qiagen DNA Stool Mini Kit protocol. Following the extraction, the DNA genotypes from fecal samples were obtained by the most powerful PCR amplification method, the IPCRp. The IPCRp-based multiplex kit amplified biotin-labeled strands we...
Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses. To describe the development and performance of the new horse genotyping kit. Methods: Highly discriminatory 17-Plex horse genotyping kit was designed by adding the fifth dye to the StockMarks kit for genotyping horses and taking advantage of the new instrument platforms. This was accomplished by using a new set of five fluorescent dyes developed by Applied Biosystems (DS-31), with four of the dyes used to label the forward amplification primers (6-FAM, VIC, NED, and PET) in each primer set. Results: The new equine kit contained five extra loci (ASB17, LEX3, HMS1, CA425, and ASB23) in addition ...