Current gene therapy.
Publisher:
Bentham Science Publishers,. Saif Zone, Sharjah, U.A.E. : Bentham Science Publishers
Frequency: Bimonthly
Country: United Arab Emirates
Language: English
Start Year:2001 -
ISSN:
1566-5232 (Print)
1875-5631 (Electronic)
1566-5232 (Linking)
1875-5631 (Electronic)
1566-5232 (Linking)
Impact Factor
3.6
| NLM ID: | 101125446 |
| (OCoLC): | 48623855 |
| LCCN: | 2001243433 |
| Classification: | W1 CU788JAFM |
AAV-mediated Overexpression of IL-10 Mitigates the Inflammatory Cascade in Stimulated Equine Chondrocyte Pellets. Following joint trauma, a posttraumatic inflammatory cascade drives degeneration of the joint. We aimed to assess whether transduction of chondrocytes with AAV5 overexpressing the immunomodulatory cytokine IL-10 would have protective effects in pellet cultures stimulated with IL-1β. Chondrocytes were isolated from 3 healthy horses and were transduced with AAV5-IL-10 at a dose of 1 x 105vg/cell. Chondrocyte pellets were formed by centrifugation and were stimulated with IL-1β starting 48 hours following transduction. After 2, 6 and 14 days in culture, supernatants were collected for cytokine a...