The Egyptian journal of immunology
Publisher:
Modern Commercial Press,
Frequency: Semiannual
Country: Egypt
Language: English
Author(s):
Egyptian Association of Immunologists.
Start Year:199u -
Identifiers
| ISSN: | 1110-4902 (Print) |
| NLM ID: | 9816016 |
| (DNLM): | SR0090435(s) |
| (OCoLC): | 50079140 |
| Coden: | EJIMFK |
| LCCN: | 2002243317 |
| Classification: | W1 EG913LLC |
Molecular detection of Babesia equi in infected and carrier horses by polymerase chain reaction. Twenty-three blood samples were used in this study; five were from five naturally infected horses with Babesia equi (B. equi), while eighteen were from asymptomatic horses with equine babesiasis from different localities in Egypt. All samples were subjected to microscopic examination, indirect fluorescent antibody test (IFA) and polymerase chain reaction (PCR). The carrier animals were microscopically detected in 7 out of 18 samples (38.8%) and in 9 of 18 by using IFA (50%), whereas PCR revealed that 14 samples were positive (78%). Two synthetic oligonucleotide primers, based on the B. equi me...