European journal of endocrinology
Publisher:
Scandinavian University Press,. Oxford : Oxford University Press (2023)
Frequency: Monthly
Country: England
Language: English
Author(s):
European Federation of Endocrine Societies.
Start Year:1994 -
ISSN:
0804-4643 (Print)
1479-683X (Electronic)
0804-4643 (Linking)
1479-683X (Electronic)
0804-4643 (Linking)
Impact Factor
5.8
| NLM ID: | 9423848 |
| (DNLM): | SR0079125(s) |
| (OCoLC): | 29970781 |
| Coden: | EJOEEP |
| LCCN: | sn 94029245 |
| Classification: | W1 EU72DDM |
Identification of amino-acids in the alpha-subunit first and third loops that are crucial for the heterospecific follicle-stimulating hormone activity of equid luteinizing hormone/choriogonadotropin. To identify amino-acids in the alpha-subunit important for expression of heterospecific FSH activity of horse (e) LH/choriogonadotropin (CG) (eLH) and donkey (dk) LH/CG (dkLH) (FSH/LH ratio ten times higher for eLH than for dkLH); this FSH activity absolutely requires an equid (donkey or horse) alpha-subunit combined with an equid beta-LH subunit. Methods: Chimeric alpha-subunits possessing the first 63 amino-acids of the porcine (p) and the last 33 amino-acids of the donkey alpha-subunit (alphap-dk) and the inverse (alphadk-p) were constructed. Porcine-specific amino-acids were introduced by ...