Experimental hematology.
Publisher:
Munksgaard.. Amsterdam : Elsevier Science Inc. (1999)
Frequency: Twelve no. a year, 1999-
Country: Netherlands
Language: English
Author(s):
International Society for Experimental Hematology.
Start Year:1973 -
ISSN:
0301-472X (Print)
1873-2399 (Electronic)
0301-472X (Linking)
1873-2399 (Electronic)
0301-472X (Linking)
Impact Factor
2.6
2022
| NLM ID: | 0402313 |
| (DNLM): | E19420000(s) |
| (OCoLC): | 01260939 |
| Coden: | EXHMA6 |
| LCCN: | sc 77000221 |
| Classification: | W1 EX504K |
Variability in the cleavage of exosome-associated transferrin receptor questions the utility of clinically useful soluble transferrin receptor assays for dogs, cats, and horses. Whole transferrin receptor (TfR) is present in reticulocyte exosomes. Soluble transferrin receptor (sTfR) is cleaved from whole TfR in human plasma, with the remnant cytoplasmic domain (cTfR) remaining membrane associated. In humans, sTfR is a biomarker that can detect iron deficiency in the presence of inflammatory disease. This condition is still a diagnostic dilemma in veterinary species. We aimed to (1) confirm the presence of exosomes and exosome-associated TfR in the serum of dogs, cats, and horses; and (2) to assess and compare the proportion of cTfR to total (cTfR + whole) in exoso...
Stromal cells from human long-term marrow cultures, but not cultured marrow fibroblasts, phagocytose horse serum constituents: studies with a monoclonal antibody that reacts with a species-specific epitope common to multiple horse serum proteins. This report describes an IgG1 mouse monoclonal antibody derived after immunization of mice with washed stromal cells from human, long-term bone marrow cultures. The antigen recognized by the antibody (BMS-1) is a carbohydrate-containing prosthetic group that is common to and specific for multiple horse serum proteins. These proteins are avidly ingested by stromal cells and concentrated in endocytic vesicles. Cultured smooth muscle cells took up the horse proteins in a similar manner to marrow stromal cells while cultured marrow fibroblasts, endothelial cells, and hepatoma cells did not. These ...