FEMS microbiology ecology.
Publisher:
Elsevier Science Publishers on behalf of the Federation of European Microbiological Societies,. Oxford Oxford University Press (2015)
Frequency: Monthly, 1993-
Country: England
Language: English
Author(s):
Federation of European Microbiological Societies.
Start Year:1985 -
ISSN:
0168-6496 (Print)
1574-6941 (Electronic)
0168-6496 (Linking)
1574-6941 (Electronic)
0168-6496 (Linking)
Impact Factor
4.2
2022
| NLM ID: | 8901229 |
| (DNLM): | SR0064668(s) |
| (OCoLC): | 11822491 |
| Coden: | FMECEZ |
| LCCN: | sn 93015705 |
| Classification: | W1 FE549BH |
A comparison of the microbiome and the metabolome of different regions of the equine hindgut. The microbiome and associated metabolome of faecal samples were compared to those from the caecum and right dorsal colon of horses and ponies euthanised for nonresearch purposes by investigating the microbial population community structure as well as their functional metabolic products. Through the use of 16S rRNA gene dendrograms, the caecum microbiome was shown to cluster separately from the other gut regions. 16S rRNA gene-based quantitative PCR (q-PCR) also demonstrated differences between the caecum and the other gut regions. Metabolites as identified by Fourier transform infrared cluster...
Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR. Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed and applied to compare the composition of intestinal microbiota of these two groups. Primers were designed to quantify the 16S rRNA gene from dominant and subdominant bacterial groups. TaqMan probes were defined for the qPCR technique used ...
Design and evaluation of group-specific oligonucleotide probes for quantitative analysis of intestinal ecosystems: their application to assessment of equine colonic microflora. Nine oligonucleotide probes complementary to conserved regions of small subunit rRNA from phylogenetically defined clusters of intestinal anaerobic bacteria were designed and evaluated for use in quantitative analysis of intestinal microflora. Optimum wash temperatures (T(w)) were determined according to the temperature of dissociation (T(d)) of each probe and target group specificity was demonstrated by comparing hybridisation to target and non-target rRNA immobilised on nylon membranes. Three probes are targeted to phylogenetic clusters of Clostridiaceae, clusters III, IV and IX, with three ...