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Immunology letters.

Periodical
Allergy and Immunology
Publisher:
Elsevier/North-Holland Biomedical Press.
Frequency: Fifteen no. a year
Country: Netherlands
Language: English
Start Year:1979 -
ISSN:
0165-2478 (Print)
1879-0542 (Electronic)
0165-2478 (Linking)
Impact Factor
4.4
2022
NLM ID:7910006
(DNLM):I06230000(s)
(OCoLC):05434613
Coden:IMLED6
LCCN:sn 80002771
Classification:W1 IM53D
Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity.
Immunology letters    May 1, 1982   Volume 4, Issue 5 231-235 doi: 10.1016/0165-2478(82)90043-8
Bártek J, Viklický V, Franĕk F, Angelisová P, Dráber P, Jarosíková T, Nĕmec M, Verlová H.Five stable hybridoma lines were prepared using the myeloma cell line P3-X63-Ag.653 and spleen cells of mice hyperimmunized by pig transferrin. All hybridomas grew well in mouse peritoneal cavity and produced antibodies of the IgG1 subclass. Antibody preparations obtained from ascitic fluids tested for their capacity of antigen precipitation. No precipitation was obtained with single antibodies and with pairs of antibodies. Three out of 10 possible triads gave clear and sharp precipitation zones and rings in immunodiffusion tests performed in agar gel. All 5 antibodies were shown by quantitati...