In vitro.
Discontinued
Publisher:
Tissue Culture Assn. [etc.]
Frequency: Thirteen no. a year
Country: United States
Language: English
Author(s):
Tissue Culture Association.
Start Year:1965 - 1984
Identifiers
| ISSN: | 0073-5655 (Print) 0073-5655 (Linking) |
| NLM ID: | 0063733 |
| (DNLM): | I06840000(s) |
| (OCoLC): | 01752709 |
| Coden: | ITCSAF |
| LCCN: | 73023797 |
| Classification: | W1 IN106 |
Spermidine cytotoxicity in vitro: effect of serum and oxygen tension. Plasma amine oxidase activities (benzylamine oxidase and spermine oxidase) were determined in the sera of a number of species of various ages. Benzylamine oxidase (BZO) activity, measured spectrophotometrically, was present in bovine, equine, and ovine species examined. Generally its activity in serum increased with the age of the animal. Spermine oxidase activity (SPO) was estimated by a bioassay of in vitro toxicity and did not necessarily correlate with BZO. Cytotoxicity in the presence of spermidine was found only in the sera of the ruminant species examined. Serum activity tended to rise ...
Proliferation and morphology of chick embryo cells cultured in the presence of horse serum and hemoglobin. We have shown previously that hemoglobin greatly stimulates chick embryo cell proliferation in Eagle's minimal essential medium supplemented with horse serum. In the present study we compared the effects of horse serum plus 10 micrometers hemoglobin to those of fetal bovine serum on subcultures of chick embryo cells serially propagated at high cell densities. The cells became elongated in the presence of fetal bovine serum and their rate of proliferation progressively decreased, whereas they became polygonal in the presence of horse serum plus hemoglobin and proliferated well in successive cel...