International journal of environmental analytical chemistry.
Publisher:
Gordon and Breach.. London : Taylor & Francis
Frequency: Fifteen no. a year,
Country: England
Language: English
Start Year:1971 -
ISSN:
0306-7319 (Print)
1029-0397 (Electronic)
0306-7319 (Linking)
1029-0397 (Electronic)
0306-7319 (Linking)
Impact Factor
2.6
2022
| NLM ID: | 0331757 |
| (DNLM): | I26780000(s) |
| (OCoLC): | 01796569 |
| Coden: | IJEAA3 |
| LCCN: | 74648494 |
| Classification: | W1 IN766GK |
A detection tube for cholinesterase inhibiting compounds. The enzyme butyrylcholinesterase from horse serum catalyses the hydrolysis of certain esters. The orange-red 2,6-dichloroindophenyl acetate will be converted by the enzyme into a deep blue alcohol. The colour transformation does not occur when the enzyme is inactivated. By making use of this biochemical reaction a cheap and simple, but very sensitive and specific detection tube could bedeveloped. The tube comprises a breakable ampoule with an aqueous buffer solution, a freeze-dried preparation of the chromogenic ester with a filler promoting its dissolution, a freeze-dried preparation of butyr...