Journal of cell science.
Publisher:
Company of Biologists. Cambridge : Company of Biologists
Frequency: Twenty four no. a year, 1997-
Country: England
Language: English
Start Year:1966 -
ISSN:
0021-9533 (Print)
1477-9137 (Electronic)
0021-9533 (Linking)
1477-9137 (Electronic)
0021-9533 (Linking)
Impact Factor
4
2022
| NLM ID: | 0052457 |
| (DNLM): | J15480000(s) |
| (OCoLC): | 01754489 |
| Coden: | JNCSAI |
| Classification: | W1 JO578S |
Glyceraldehyde 3-phosphate dehydrogenase is bound to the fibrous sheath of mammalian spermatozoa. Evidence is provided that the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase is covalently linked to the fibrous sheath. The fibrous sheath is a typical structure of mammalian spermatozoa surrounding the axoneme in the principal piece of the flagellum. More than 90% of boar sperm glyceraldehyde 3-phosphate dehydrogenase activity is sedimented after cell disintegration by centrifugation. Detergents, different salt concentrations or short term incubation with chymotrypsin do not solubilize the enzyme, whereas digestion with trypsin or elastase does. Short term incubation with trypsin...
Adaptation of human diploid fibroblasts in vitro to serum from different sources. The growth of two human diploid skin fibroblast cell lines, originally grown in medium supplemented with foetal bovine serum and later adapted to medium supplemented with newborn bovine, bovine calf or horse serum, has been studied. Prolonged generation times increased cell volumes and decreased plating efficiencies were observed in cultures grown in newborn bovine, bovine calf or horse serum. In general, the deleterious effects were most severe as a result of growth in bovine calf or horse serum. In the light of the present findings, we believe investigators should exert great caution in swit...