Journal of neuroscience research.
Publisher:
Liss.. New York, NY : Wiley Interscience
Frequency: Monthly, 2011-
Country: United States
Language: English
Start Year:1975 -
ISSN:
0360-4012 (Print)
1097-4547 (Electronic)
0360-4012 (Linking)
1097-4547 (Electronic)
0360-4012 (Linking)
Impact Factor
4.2
2022
| NLM ID: | 7600111 |
| (DNLM): | J31220000(s) |
| (OCoLC): | 02244695 |
| Coden: | JNREDK |
| Classification: | W1 JO795C |
GRASP: a novel heparin-binding serum glycoprotein that mediates oligodendrocyte-substratum adhesion. Cell-substratum adhesion plays a crucial part in the cascade of events that control growth or turn on and consummate a differentiation program. We are investigating the molecular basis of oligodendrocyte (OLG) cytodifferentiation, employing pure cultures of OLGs isolated from postmyelination brains. We have shown that such OLGs will regenerate in vitro and reenact the ontogenic development of myelin, but to do so they need a signal. Adherence to a polylysine surface in the presence of 20% horse serum generates such a signal. Among the events that are turned on upon OLG adhesion is the phosphor...
Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin. We have previously shown that neuroblasts from cerebral hemispheres of 6-day-old chick embryos are able to proliferate when grown in the presence of fetal calf serum. We report here that in the presence of horse serum alone the proliferative rate of neuroblasts is strongly reduced. A high proliferative rate is restored upon the addition of bovine transferrin and to a lesser extent with added FeSO4 or hemin. These findings suggest that the transferrin of horse serum cannot be used by chick neuroblasts in vitro, while bovine transferrin exogenously added is active in promoting cell proliferation...