Journal of physiology and biochemistry.
Publisher:
Servicio de Publicaciones de la Universidad De Navarra (departamentos de fisiología y nutrición y de bioquimica),
Frequency: Quarterly
Country: Spain
Language: English
Start Year:1998 -
ISSN:
1138-7548 (Print)
1877-8755 (Electronic)
1138-7548 (Linking)
1877-8755 (Electronic)
1138-7548 (Linking)
Impact Factor
3.4
2022
| NLM ID: | 9812509 |
| (DNLM): | SR0093356(s) |
| (OCoLC): | 39695269 |
| Coden: | JPBIF2 |
| LCCN: | 98655842 |
| Classification: | W1 RE534 |
Influence of epidermal growth factor on mammalian oocyte maturation via tyrosine-kinase pathway. Epidermal growth factor (EGF) has been reported to promote different functions in mammalian ovaries, including oocyte maturation. The aim of the present study was to establish: that EGF influences oocyte maturation in ovine and equine, that a tyrosine kinase-dependent intracellular mechanism mediates EGF effect and, that EGF-R receptor is detectable in ovarian follicles by immunohistochemistry methods. Selected ovine and equine oocytes were aspirated from 2-5 mm (ovine) or 25 mm (equine) follicles and cultured in TCM 199 for 22 (ovine) or 36 hours (equine). They are then subjected to culture w...
Influence of epidermal growth factor on mammalian oocyte maturation via tyrosine-kinase pathway. Epidermal growth factor (EGF) has been reported to promote different functions in mammalian ovaries, including oocyte maturation. The aim of the present study was to establish: that EGF influences oocyte maturation in ovine and equine, that a tyrosine kinase-dependent intracellular mechanism mediates EGF effect and, that EGF-R receptor is detectable in ovarian follicles by immunohistochemistry methods. Selected ovine and equine oocytes were aspirated from 2-5 mm (ovine) or 25 mm (equine) follicles and cultured in TCM 199 for 22 (ovine) or 36 hours (equine). They are then subjected to culture w...