Japanese journal of infectious diseases.
Publisher:
National Institute of Infectious Diseases,
Frequency: Bimonthly
Country: Japan
Language: English
Author(s):
Kokuritsu Kansenshō Kenkyūjo (Japan)
Start Year:1999 -
ISSN:
1344-6304 (Print)
1884-2836 (Electronic)
1344-6304 (Linking)
1884-2836 (Electronic)
1344-6304 (Linking)
Impact Factor
2.2
| NLM ID: | 100893704 |
| (OCoLC): | 41656046 |
| Classification: | W1 JA975 |
Venom and Antivenom of the Redback Spider (Latrodectus hasseltii) in Japan. Part I. Venom Extraction, Preparation, and Laboratory Testing. The redback spider (Latrodectus hasseltii Thorell) reportedly invaded Japan in September 1995. To date, 84 redback spider bite cases have been reported; 7 of these cases employed the antivenom. Antivenom has been imported from Australia in the past, but because of restrictions on exportation it was evident that nearly all of the antivenom present in Japan would expire during 2014. In 2014, a plan was proposed to experimentally manufacture and stockpile a horse antiserum for ourselves, using redback spiders indigenous to Japan. A total of 11,403 female spiders were captured alive: 1,217 from th...
Venom and Antivenom of the Redback Spider (Latrodectus hasseltii) in Japan. Part II. Experimental Production of Equine Antivenom against the Redback Spider. This is the first report on large-scale experimental production of an equine antivenom against the redback spider (Latrodectus hasseltii) lived in Japan. We captured 10,000 redback spiders in Japan and prepared the toxoids of crude venom extract, mixed the toxoids with a mineral oil adjuvant, and immunized healthy horses repeatedly over a period of several weeks. Thereafter, we separated the horse plasma, purified the γ-globulin fraction, and stocked it as a purified antivenom concentrate. Consequently, we manufactured approximately 6,500 vials of a single-dose freeze-dried test lot from a po...