Klinicheskaia laboratornaia diagnostika.
Publisher:
"Meditsina",
Frequency: Monthly, 1997-
Country: Russia (Federation)
Language: Russian
Author(s):
Soi︠u︡z nauchnykh obshchestv klinicheskoĭ laboratornoĭ diagnostiki., Russia (Federation). Ministerstvo zdravookhranenii︠a︡., Russia (Federation). Ministerstvo zdravookhranenii︠a︡ i medit︠s︡inskoĭ promyshlennosti.
Start Year:1992 -
Identifiers
| ISSN: | 0869-2084 (Print) 2412-1320 (Electronic) 0869-2084 (Linking) |
| NLM ID: | 9432021 |
| (DNLM): | SR0077292(s) |
| (OCoLC): | 30431410 |
| Coden: | KLDIES |
| Classification: | W1 KL186 |
[Comparative analysis of LAMP and Real Time PCR methods to detect pathogens of glanders and meliodosis.]. Results of detection of Burkholderia mallei and Burkholderia pseudomallei DNA strains by LAMP (Loop-mediated Isothermal Amplification) and Real Time PCR are shown. It has been revealed that, in Real Time PCR, primers steadily detected DNA of those microorganism for the sequences of which they were designed. The above mentioned primers did not detect DNA of heterologous strains. During LAMP method no set of primers showed high analytical sensitivity and specificity. Primers did not detected DNA of all the strains under research to target genes of which they were not intended, but they were capa...