Systems biology in reproductive medicine.
Publisher:
Informa Healthcare. London : Informa Healthcare
Frequency: Bimonthly
Country: England
Language: English
Start Year:2008 -
ISSN:
1939-6368 (Print)
1939-6376 (Electronic)
1939-6368 (Linking)
1939-6376 (Electronic)
1939-6368 (Linking)
Impact Factor
2.4
2022
| NLM ID: | 101464963 |
| (OCoLC): | 166267843 |
| LCCN: | 2007212290 |
| Classification: | W1 AR436 |
High throughput deep proteomic analysis of seminal plasma from stallions with contrasting semen quality. Seminal plasma proteins and pathways associated with sperm motility have not been elucidated in stallions. Therefore, in the current study, using the high throughput LC/MS-MS approach, we profiled stallion seminal plasma proteins and identified the proteins and pathways associated with sperm motility. Seminal plasma from six stallions producing semen with contrasting sperm motility ( = 3 each high-and low-motile group) was utilized for proteomic analysis. We identified a total of 1687 proteins in stallion seminal plasma, of which 1627 and 1496 proteins were expressed in high- (HM) and low-...
Biochemical characterization of stallion prostasomes and comparison to their human counterparts. Release of nanometer-sized prostasomes into human and equine semen suggests essential functions in their relationships with sperm cells and the fertilization process. The two types of prostasomes displayed ultrastructural similarities, albeit the human prostasomes were somewhat larger than the stallion prostasomes. A high ratio of saturated fatty acids was characteristic for the two prostasome types. Electrophoretic separation systems revealed an equine prostasomal pattern different from that of human. The 21 distinctive low molecular weight protein spots in the 2D-gel (with no counterparts in...