Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases.
Publisher:
Zhongguo yu fang yi xue ke xue yuan Ji sheng chong bing yan jiu suo, 1987-. Shanghai : Institute Of Parasitic Diseases, Chinese Academy Of Preventive Medicine
Frequency: Four no. a year
Country: China
Language: chi
Author(s):
Zhongguo yu fang yi xue ke xue yuan. Ji sheng chong bing yan jiu suo.
Start Year:1987 -
Identifiers
| ISSN: | 1000-7423 (Print) 1000-7423 (Linking) |
| NLM ID: | 8709992 |
| (DNLM): | SR0060573(s) |
| (OCoLC): | 16742612 |
| Classification: | W1 CH986H |
[An indirect ELISA for the detection of Babesia caballi in equine animals]. To clone and express BC48 gene of Babesia caballi, and to establish an indirect ELISA for the diagnosis of B. caballi in equine animals. Methods: The genomic DNA of B. caballi was extracted from the infected donkey blood. BC48 gene was amplified by PCR. The PCR product was cloned into expression plasmid pET28a, and expressed in E. coli BL21 with IPTG induction. The recombinant protein was purified by Ni-NTA affinity chro-matography and was used as a diagnostic antigen to establish an indirect ELISA. The reaction conditions of the indirect ELISA were optimized. Specificity and sensitivity of th...
Relationship of the livestock trade to schistosomiasis transmission in mountainous area. To study the relationship between the livestock trade and schistosomiasis transmission and to provide an evidence for making a strategy of schistosomiasis control in mountainous areas. Methods: A retrospective survey and analysis was conducted to investigate the prevalence of schistosomiasis in both humans and livestock (cattle, horses, mules, donkeys and pigs), and the number and migration of livestock in Weishan County, Yunnan Province in 1980-1991. Results: A positive correlation was found between the infection rate of residents and the numbers of livestock migration (R = 0.9151, P < 0.0...