Plasma clearance and half-life of prostaglandin F2alpha: a comparison between mares and heifers.
Abstract: Horses are about five times more sensitive to the luteolytic effect of prostaglandin F2alpha (PGF) than cattle, as indicated by a recommended clinical dose of 5 mg in horses and 25 mg in cattle. Novel evaluations of the PGF plasma disappearance curves were made in mares and in heifers, and the two species were compared. Mares and heifers (n = 5) of similar body weight were injected (Min 0) intravenously with PGF (5 mg per animal). Blood was sampled every 10 sec until Min 3, every 30 sec until Min 5, every 10 min until Min 60, and every 30 min until Min 240. The mean PGF concentration was greater (P < 0.05) in mares than in heifers at Min 1 through Min 60 and at Mins 180 and 240. The mean time to maximum PGF concentration was not different between mares (42.0 ± 8.6 sec) and heifers (35.0 ± 2.9 sec). The apparent plasma clearance, distribution half-life, elimination half-life, and maximum plasma PGF concentration were 3.3 ± 0.5 L h(-1) kg(-1), 94.2 ± 15.9 sec, 25.9 ± 5.0 min, and 249.1 ± 36.8 ng/ml, respectively, in mares and 15.4 ± 2.3 L h(-1) kg(-1), 29.2 ± 3.9 sec, 9.0 ± 0.9 min, and 51.4 ± 22.6 ng/ml, respectively, in heifers. Plasma clearance was about five times less (P < 0.0005), maximum plasma PGF concentration was five times greater (P < 0.002), and the distribution half-life and elimination half-life were about three times longer (P < 0.005) in mares than in heifers. The fivefold greater plasma clearance of PGF in heifers than in mares corresponds to the recommended fivefold greater clinical dose of PGF in cattle and supported the hypothesis that the metabolic clearance of PGF is slower in mares than heifers.
Publication Date: 2012-07-19 PubMed ID: 22553220DOI: 10.1095/biolreprod.112.100776Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study explores the differences in the plasma clearance and half-life of prostaglandin F2alpha (PGF) in horses and cattle, stating that horses appear to be more sensitive to this substance, as indicated by the dosage recommendations for each species.
Study Background
- The study was predicated on the fact that horses appear to require lower doses of PGF – a type of naturally occurring fat that has hormone-like effects – than cattle. Specifically, while the typical recommended dose for horses is around 5 mg, for cattle it is about 25 mg, suggesting a fivefold disparity in responses to this substance.
Methodology
- To investigate these differences further, the researchers conducted a comparative study using horses (mares) and cattle (heifers), each group comprised of five members with similar body weights. Both groups were intravenously administered a 5mg dose of PGF.
- Blood samples were then taken at various intervals up to 240 minutes post-injection in order to track the PGF plasma disappearance and map it onto corresponding curves.
Findings
- Across the duration of the experiment, particularly between the 1- and 60-minute marks, and again at the 180- and 240-minute marks, the concentration of PGF in the plasma of the mares was found to be notably higher compared to the heifers, according to scientific statistical significance (P less than 0.05).
- The maximum PGF concentration was achieved at similar times for the mares and heifers, but various significant disparities between the two were noted. Specifically, plasma clearance was approximately five times slower, plasma PGF concentration was around five times greater, and both distribution half-life and elimination half-life were about three times longer in mares than in heifers.
Conclusions
- The differential response rate between the species, with mares demonstrating a slower metabolic clearance than heifers, validates the current discrepancy in PGF dosage recommendations.
- Understanding these physiological variations between species can help veterinary physicians optimize therapeutic strategies and dosage levels for the use of PGF in mares and heifers.
Cite This Article
APA
Shrestha HK, Beg MA, Burnette RR, Ginther OJ.
(2012).
Plasma clearance and half-life of prostaglandin F2alpha: a comparison between mares and heifers.
Biol Reprod, 87(1), 18-6.
https://doi.org/10.1095/biolreprod.112.100776 Publication
Researcher Affiliations
- Eutheria Foundation, Cross Plains, Wisconsin, USA.
MeSH Terms
- Animals
- Cattle / blood
- Dinoprost / administration & dosage
- Dinoprost / blood
- Dinoprost / pharmacokinetics
- Female
- Half-Life
- Horses / blood
- Luteolytic Agents / administration & dosage
- Luteolytic Agents / blood
- Luteolytic Agents / pharmacokinetics
- Metabolic Clearance Rate
- Species Specificity
Citations
This article has been cited 8 times.- Piotrowska-Tomala KK, Jonczyk AW, Szóstek-Mioduchowska AZ, Żebrowska E, Ferreira-Dias G, Skarzynski DJ. The Effects of Prostaglandin E(2) Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study. Front Vet Sci 2021;8:753796.
- Pemayun TGO, Mustofa I, Mahaputra L, Hermadi HA, Wijaya NMR, Mulyati S, Utama S, Restiadi TI, Rimayanti R. Fertility restoration of racing mare with persistent corpus luteum. Vet World 2021 Sep;14(9):2356-2361.
- Rebordão MR, Amaral A, Fernandes C, Silva E, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF(2α) Pathway in the Mare Endometrium. Animals (Basel) 2021 Sep 6;11(9).
- Zhang Z, Emami S, Hennebelle M, Morgan RK, Lerno LA, Slupsky CM, Lein PJ, Taha AY. Linoleic acid-derived 13-hydroxyoctadecadienoic acid is absorbed and incorporated into rat tissues. Biochim Biophys Acta Mol Cell Biol Lipids 2021 Mar;1866(3):158870.
- Morgenstern J, Fleming T, Kadiyska I, Brings S, Groener JB, Nawroth P, Hecker M, Brune M. Sensitive mass spectrometric assay for determination of 15-deoxy-Δ(12,14)-prostaglandin J(2) and its application in human plasma samples of patients with diabetes. Anal Bioanal Chem 2018 Jan;410(2):521-528.
- Chuang AC, Ho TC, Chan JP. Prostaglandin-induced luteolysis and estrous expression in dairy heifers: applications of ovulation prediction based on automated activity monitoring system. J Vet Med Sci 2025 Sep 1;87(9):1005-1011.
- Armansyah T, Husnurrizal H, Wahyuni S, Hafizuddin H, Siregar TN, Sutriana A, Sayuti A, Syahrani AT, Pariansyah MB. Direct and indirect effects of PGF2α administration in male Wistar rats based on increased expression of α-SMA and androgen receptor. Open Vet J 2024 Dec;14(12):3474-3486.
- Zhu X, Zhang X, Zhang Y, Li J, Li S, Zhang S, Li L, Meng L, Wei H, Zhang S. Cloprostenol sodium improves reproductive performance of multiparous sows during lactation. Front Vet Sci 2024;11:1342930.
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