Chemiluminescent assays are analytical techniques used to measure the presence and concentration of specific substances in biological samples through the emission of light during a chemical reaction. In equine research, these assays are employed to detect and quantify various biomarkers, hormones, and other analytes in horse blood, serum, or other tissues. The method involves a luminescent reaction that occurs when a substrate interacts with an enzyme or other reactant, producing light that is measured by a luminometer. This technique is valued for its sensitivity, specificity, and ability to provide quantitative results. This page compiles peer-reviewed research studies and scholarly articles that explore the application, methodology, and findings of chemiluminescent assays in equine health and diagnostics.
Asbury AC, Schultz KT, Klesius PH, Foster GW, Washburn SM.Ten mares, 5 resistant and 5 susceptible to bacterial endometritis, were examined for differences pertaining to the efficiency of phagocytosis of bacteria by neutrophils in the uterus. An assay for chemiluminescence was used to evaluate various schemes of opsonization and relate them to phagocytic rate. 123I-labelled albumin was used to measure protein migration to the inoculated uterus. Neutrophil numbers moving to the uterine lumen were determined. Before inoculation resistant mares were found to have substances in their uterine secretions that opsonized bacteria, resulting in effective phag...
Durham AE.This study aimed to further define and quantify possible cross-reactive peptides when measuring plasma adrenocorticotropic hormone (ACTH) concentration in equids. Equine plasma samples were spiked with known concentrations of exogenous manufactured peptides comprising human ACTH, ACTH (corticotropin-like intermediate lobe peptide, CLIP) and ACTH (corticotropin inhibiting peptide, CIP). All samples were assayed in duplicate using Siemens Immulite 2000xpi chemiluminescent assay (CLA) and Tosoh AIA-900 immunoflurorescent assay (IFA). As expected, ACTH was measured by both assays although higher v...