Analyze Diet

Topic:Chemiluminescent Assay

Chemiluminescent assays are analytical techniques used to measure the presence and concentration of specific substances in biological samples through the emission of light during a chemical reaction. In equine research, these assays are employed to detect and quantify various biomarkers, hormones, and other analytes in horse blood, serum, or other tissues. The method involves a luminescent reaction that occurs when a substrate interacts with an enzyme or other reactant, producing light that is measured by a luminometer. This technique is valued for its sensitivity, specificity, and ability to provide quantitative results. This page compiles peer-reviewed research studies and scholarly articles that explore the application, methodology, and findings of chemiluminescent assays in equine health and diagnostics.
Factors affecting phagocytosis of bacteria by neutrophils in the mare’s uterus.
Journal of reproduction and fertility. Supplement    January 1, 1982   Volume 32 151-159 
Asbury AC, Schultz KT, Klesius PH, Foster GW, Washburn SM.Ten mares, 5 resistant and 5 susceptible to bacterial endometritis, were examined for differences pertaining to the efficiency of phagocytosis of bacteria by neutrophils in the uterus. An assay for chemiluminescence was used to evaluate various schemes of opsonization and relate them to phagocytic rate. 123I-labelled albumin was used to measure protein migration to the inoculated uterus. Neutrophil numbers moving to the uterine lumen were determined. Before inoculation resistant mares were found to have substances in their uterine secretions that opsonized bacteria, resulting in effective phag...