Topic:Immunoblotting
Immunoblotting, also known as Western blotting, is a laboratory technique used to detect specific proteins in a sample. In equine research, this method is applied to analyze protein expression and modifications in horses, contributing to the understanding of various biological processes and disease states. Immunoblotting involves the separation of proteins by gel electrophoresis, followed by their transfer to a membrane and subsequent detection using specific antibodies. This technique is utilized in studies investigating equine physiology, pathology, and immunology, providing insights into protein-related functions and interactions. This page compiles peer-reviewed research studies and scholarly articles that explore the application, methodology, and findings of immunoblotting in equine science.
Characterization of the serological cross-reactivity between glycoproteins of the human immunodeficiency virus and equine infectious anaemia virus. The reported serological relatedness between the major glycoproteins of human immunodeficiency virus (HIV gp120) and equine infectious anaemia virus (EIAV gp90) was examined using purified antigens in radioimmunoprecipitation (RIP), radioimmunoassay (RIA) and immunoblot assays with reference serum from acquired immunodeficiency syndrome (AIDS) patients, an anti-gp120 goat serum and EIAV-infected horse serum. To assess the contributions of glycoprotein oligosaccharide and peptide components to any observed reactivities, antigens treated with endoglycosidase F to remove carbohydrate were assayed...
Characteristics of an R antigen common to Streptococcus equi and zooepidemicus. An R antigen of the group C streptococcus S. equi that cross reacts with a similar antigen of S. zooepidemicus has been identified and characterized. It is acid, heat and trypsin resistant, but pepsin sensitive and has an isoelectric point of 4.8. The amino acids in highest concentration are glutamic, aspartic, alanine, leucine, and valine. Bacterial components released in a French Press contain large amounts of R antigen, which is present also in culture supernatants and acid extracts. It has a molecular weight of about 82,000. Trypsin extraction of cells yields molecules of predominantly 56,...