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Topic:Misuse
The topic of misuse in regards to horses encompasses the inappropriate or unethical treatment and management practices that can negatively impact equine welfare. This includes practices such as overuse of performance-enhancing drugs, improper training methods, neglect, and inadequate living conditions. Misuse can lead to physical and psychological harm, affecting the overall health and performance of horses. Research in this area often focuses on identifying the prevalence and effects of such practices, as well as developing guidelines and interventions to prevent misuse. This page gathers peer-reviewed research studies and scholarly articles that explore the various aspects of misuse in equine management, including its identification, consequences, and strategies for prevention and education.
Gene Doping Control Analysis of Human Erythropoietin Transgene in Equine Plasma by PCR-Liquid Chromatography High-Resolution Tandem Mass Spectrometry. Gene doping involves the misuse of genetic materials to alter an athlete's performance, which is banned at all times in both human and equine sports. Quantitative polymerase chain reaction (qPCR) assays have been used to control the misuse of transgenes in equine sports. Our laboratory recently developed and implemented duplex as well as multiplex qPCR assays for transgenes detection. To further advance gene doping control, we have developed for the first time a sensitive and definitive PCR-liquid chromatography high-resolution tandem mass spectrometry (PCR-LC-HRMS/MS) method for transgene det...
Control of the misuse of bromide in horses. Bromide is a sedative hypnotic. Due to its potential use as a sedative or calmative agent in competition horses, a method to control bromide is needed. Colorimetric method had been employed in the authors' laboratory from 2003 for the semi-quantification of bromide in equine plasma samples. However, the method was found to be highly susceptible to matrix interference, and was replaced in 2008 with a more reliable inductively coupled plasma-mass spectrometry (ICP/MS) method. Equine plasma was protein-precipitated using trichloroacetic acid, diluted with nitric acid, and then submitted directly ...
