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Home / Equine Research Bank / Curr Microbiol / 16S rDNA and ITS Sequence Diversity of Burkholderia mallei I...
Current microbiology2021; 79(1); 31; doi: 10.1007/s00284-021-02701-8

16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019).

Abstract: Glanders is a highly contagious and fatal infection of equids caused by the bacteria known as Burkholderia mallei. It is one of the notifiable equine diseases and is still present in Asia, South America and Africa. In India, glanders re-emerged in 2006, and thereafter, increasing numbers of cases were reported in different regions of the country. Between 2013 and 2019, 39 B. mallei were isolated from glanders-affected horses (n = 30) and mules (n = 9) from seven states of India such as Uttar Pradesh, Haryana, Delhi, Himachal Pradesh, Gujarat, Maharashtra and Tamil Nadu. In this study, the phylogenetic relationships of these isolates were assessed by sequence analysis of 16S rDNA gene and ITS region. Purified PCR-amplified products of 16S rDNA gene and ITS region were sequenced, aligned and phylogenetic trees were constructed using MEGA 11 software. Additionally, B. mallei 16S rDNA (n = 36) and ITS (n = 18) sequences available in the GenBank were also included for analysis to determine the diversity of older B. mallei isolates with recent Indian isolates. Both the phylogeny showed that the majority of the recent isolates from India are closely related to each other, but are genetically diverse from older isolates that originated from India. Nucleotide substitutions were also observed in a single and double position in 12 recent and two old Indian isolates. The study also indicates that similar B. mallei strains were responsible for glanders outbreaks in different states (Uttar Pradesh- Himachal Pradesh and Uttar Pradesh- Haryana) and this is due to the migration of infected animals from one state to another state. This study implies that 16S rDNA and ITS region may be used for molecular characterization of B. mallei associated with glanders in resource-limited settings.
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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Publication Date: 2021-12-18 PubMed ID: 34921617PubMed Central: 3766238DOI: 10.1007/s00284-021-02701-8Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article analyzes the genetic diversity of Burkholderia mallei, a bacteria causing a deadly equine disease called Glanders in India, using gene sequencing methods during 2013-2019.

Overview of the Research

  • The study focuses on glanders, an infectious disease caused by Burkholderia mallei bacteria, affecting horses and mules. This fatal disease has re-emerged in India, with rising cases reported from 2006 onward.
  • The research establishes the genetic relationships of 39 B. mallei isolates obtained from affected horses and mules across seven Indian states between 2013 and 2019. These states include Uttar Pradesh, Haryana, Delhi, Himachal Pradesh, Gujarat, Maharashtra, and Tamil Nadu.

Methodology of the Research

  • 16S rDNA and Internal Transcribed Spacer (ITS) genes were sequenced and processed using purified PCR-amplified products and analyzed using MEGA 11 software. This method allows for the study of the phylogenetic relationships among B. mallei isolates.
  • The researchers also paralleled their analysis with 36 B. mallei 16S rDNA sequences and 18 ITS sequences from the GenBank to compare the diversity of older B. mallei strains with recent Indian isolates.

Findings of the Research

  • The study’s results indicate that recent Indian isolates of B. mallei are genetically similar to each other but show diversity when compared to older Indian isolates. This highlights the evolving nature of pathogenic bacteria over time.
  • Nucleotide substitutions were found in some of these isolates, indicating genetic variation.
  • The research also suggested that similar B. mallei strains caused Glanders outbreaks in different states due to the transportation of infected animals from one state to another.

Conclusion of the Research

  • The study concludes that 16S rDNA and ITS region sequencing may be useful for characterizing B. mallei associated with glanders, especially in resource-limited settings. This offers a valuable tool for tracking disease spread and devising effective control strategies.

Cite This Article

APA
Shanmugasundaram K, Singha H, Saini S, Tripathi BN. (2021). 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019). Curr Microbiol, 79(1), 31. https://doi.org/10.1007/s00284-021-02701-8

Publication

Current microbiology
ISSN: 1432-0991
NlmUniqueID: 7808448
Country: United States
Language: English
Volume: 79
Issue: 1
Pages: 31

Researcher Affiliations

Shanmugasundaram, Karuppusamy
  • Indian Council of Agricultural Research-National Research Centre On Equines (ICAR-NRCE), Hisar, Haryana, 125001, India.
Singha, Harisankar
  • Indian Council of Agricultural Research-National Research Centre On Equines (ICAR-NRCE), Hisar, Haryana, 125001, India. hsssankarbty@gmail.com.
Saini, Sheetal
  • Indian Council of Agricultural Research-National Research Centre On Equines (ICAR-NRCE), Hisar, Haryana, 125001, India.
Tripathi, Bhupendra N
  • Division of Animal Sciences, Indian Council of Agricultural Research, Krishi Bhavan, New Delhi, 110 001, India. bntripathi1@yahoo.co.in.

MeSH Terms

  • Animals
  • Burkholderia mallei / genetics
  • DNA, Ribosomal / genetics
  • Equidae
  • Glanders
  • Horses
  • India
  • Phylogeny

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Citations

This article has been cited 1 times.
  1. Brangsch H, Singha H, Laroucau K, Elschner M. Sequence-based detection and typing procedures for Burkholderia mallei: Assessment and prospects.. Front Vet Sci 2022;9:1056996.
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