A 1.3-Mb interval map of equine homologs of HSA2.
Abstract: A comparative approach that utilizes information from more densely mapped or sequenced genomes is a proven and efficient means to increase our knowledge of the structure of the horse genome. Human chromosome 2 (HSA2), the second largest human chromosome, comprising 243 Mb, and containing 1246 known genes, corresponds to all or parts of three equine chromosomes. This report describes the assignment of 140 new markers (78 genes and 62 microsatellites) to the equine radiation hybrid (RH) map, and the anchoring of 24 of these markers to horse chromosomes by FISH. The updated equine RH maps for ECA6p, ECA15, and ECA18 resulting from this work have one, two, and three RH linkage groups, respectively, per chromosome/chromosome-arm. These maps have a three-fold increase in the number of mapped markers compared to previous maps of these chromosomes, and an increase in the average marker density to one marker per 1.3 Mb. Comparative maps of ECA6p, ECA15, and ECA18 with human, chimpanzee, dog, mouse, rat, and chicken genomes reveal blocks of conserved synteny across mammals and vertebrates.
2006 S. Karger AG, Basel.
Publication Date: 2006-02-18 PubMed ID: 16484777DOI: 10.1159/000089875Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research article presents a comparative study that utilizes information from densely mapped or sequenced genomes to enhance our understanding of the structure of the horse genome, specifically using human chromosome 2 (HSA2). The study reports the assignment of 140 new markers to the equine radiation hybrid (RH) map and links these markers to horse chromosomes.
Research Methodology
- The research utilizes a comparative approach that employs information from more densely mapped or sequenced genomes. This approach has proven to be a highly efficient way to increase our knowledge of the genome structure of different species.
- This study focuses on the Human chromosome 2 (HSA2), the second largest among human chromosomes. HSA2 comprises 243 Mb and contains 1246 known genes. It corresponds to all or parts of three equine chromosomes.
- The research reports the assignment of 140 new markers to the equine radiation hybrid (RH) map. These markers consist of 78 genes and 62 microsatellites. Furthermore, the study has anchored 24 of these markers to horse chromosomes through Fluorescent In Situ Hybridization (FISH).
Study Results
- The updated equine RH maps for ECA6p, ECA15, and ECA18 resulting from this study have one, two, and three RH linkage groups respectively, per chromosome/chromosome-arm.
- The research showed a three-fold increase in the number of mapped markers compared to previous maps of these chromosomes.
- There is an increase in the average marker density to one marker per 1.3 Mb.
- Comparative maps of ECA6p, ECA15, and ECA18 with human, chimpanzee, dog, mouse, rat, and chicken genomes reveal blocks of conserved synteny across mammals and vertebrates.
Implications
- This study drastically enhances our understanding of the genome structure of horses. It provides a framework for further genetic studies in horses and other equines.
- The comparative maps highlighting blocks of conserved synteny across mammals and vertebrates provide a roadmap for understanding the evolution and phylogeny of these species.
- The increase in mapped markers and the linking of these markers to horse chromosomes might prove beneficial in diagnosing and treating genetic diseases in equines.
Cite This Article
APA
Wagner ML, Raudsepp T, Goh G, Agarwala R, Schaffer AA, Dranchak PK, Brinkmeyer-Langford C, Skow LC, Chowdhary BP, Mickelson JR.
(2006).
A 1.3-Mb interval map of equine homologs of HSA2.
Cytogenet Genome Res, 112(3-4), 227-234.
https://doi.org/10.1159/000089875 Publication
Researcher Affiliations
- Department of Veterinary Biosciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.
MeSH Terms
- Animals
- Chromosome Mapping
- Chromosomes, Artificial, Bacterial
- Chromosomes, Human, Pair 2 / genetics
- Cricetinae / genetics
- DNA Primers
- Genetic Markers
- Horses / genetics
- Humans
- In Situ Hybridization, Fluorescence
- Metaphase
- Nucleic Acid Hybridization
Citations
This article has been cited 2 times.- Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee EJ, Agarwala R, Stallknecht-Rice E, Schäffer AA, Skow LC, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. A 4,103 marker integrated physical and comparative map of the horse genome. Cytogenet Genome Res 2008;122(1):28-36.
- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
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