A combination of single-drop microextraction and open tubular capillary electrochromatography with carbon nanotubes as stationary phase for the determination of low concentration of illicit drugs in horse urine.
Abstract: In this study we developed an interesting alternative to HPLC-mass spectrometry for the quantification of seven important drugs of abuse in racehorses. The procedure proposed in this work is a combination of single-drop microextraction (SDME) and an open tubular capillary electrochromatography (OT-CEC) using multi-wall carbon nanotubes (MWCTs) immobilized into a fused-silica capillary as a stationary phase. The SDME showed to be a powerful tool for extraction/preconcentration of the seven drugs analyzed in the study, showing an enrichment factor between 38- and 102-fold depending on the drug. We have investigated the electrophoretic features of MWCTs immobilized fused-silica capillary by covalent modification of the inner surface of the capillary. The results show a good run-to-run, day-to-day and capillary-to-capillary reproducibility of the method. Compared with the capillary zone electrophoresis (CZE), the coating of the capillary allowed the separation of the analytes with high resolution, with less band-broadening and without distortion of the baseline. The interactions between the analytes and the MWCTs resulted in an increased migration time and probably this was the reason of the front tailing effect. The results showed a good capillary efficiencies and an improved of the electrophoretic separation.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-09-16 PubMed ID: 22063542DOI: 10.1016/j.talanta.2011.09.014Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article presents a new method for detecting low concentrations of illegal drugs in horse urine using single-drop microextraction and open tubular capillary electrochromatography, with carbon nanotubes as the stationary phase.
Combining SDME and OT-CEC
- The researchers developed an alternative to HPLC-mass spectrometry for identifying seven key drugs of abuse in racehorses.
- They combined single-drop microextraction (SDME) with open tubular capillary electrochromatography (OT-CEC) for this process.
- Multi-wall carbon nanotubes (MWCTs) immobilized into a fused-silica capillary were used as a stationary phase in the process.
Effectiveness of SDME
- SDME proved to be an effective tool for extracting and concentrating the seven drugs analyzed in the study.
- The enrichment factor varied between 38 and 102, depending on the specific drug.
Electrophoretic Features
- The researchers assessed the electrophoretic capabilities of an immobilized MWCT fused-silica capillary.
- They implemented covalent modification on the inner capillary surface.
- This resulted in consistent reproducibility of the method across different tests, days, and capillaries.
Comparison with CZE
- Compared to capillary zone electrophoresis (CZE), the new method involving capillary coating provided superior separation of the analytes.
- This led to high-resolution results, less band-broadening, and no distortion of the baseline.
Interaction between Analytes and MWCTs
- The interaction between the analytes and the MWCTs increased the migration time, causing a front tailing effect.
- Despite this, the results indicated high capillary efficiencies and improved electrophoretic separation.
Cite This Article
APA
Stege PW, Lapierre AV, Martinez LD, Messina GA, Sombra LL.
(2011).
A combination of single-drop microextraction and open tubular capillary electrochromatography with carbon nanotubes as stationary phase for the determination of low concentration of illicit drugs in horse urine.
Talanta, 86, 278-283.
https://doi.org/10.1016/j.talanta.2011.09.014 Publication
Researcher Affiliations
- INQUISAL, Department of Analytical Chemistry, National University of San Luis, CONICET, Chacabuco y Pedernera, D5700BWS San Luis, Argentina. pwstege@unsl.edu.ar
MeSH Terms
- Animals
- Capillary Electrochromatography / methods
- Doping in Sports / prevention & control
- Horses
- Illicit Drugs / analysis
- Illicit Drugs / urine
- Liquid Phase Microextraction / methods
- Nanotubes, Carbon / chemistry
Citations
This article has been cited 2 times.- Ahn S, Bae S. Synthesis and Characterization of a Multi-Walled Carbon Nanotube-Ionic Liquid/Polyaniline Adsorbent for a Solvent-Free In-Needle Microextraction Method. Molecules 2023 Apr 17;28(8).
- Ahmad SM, Gonçalves OC, Oliveira MN, Neng NR, Nogueira JMF. Application of Microextraction-Based Techniques for Screening-Controlled Drugs in Forensic Context-A Review. Molecules 2021 Apr 9;26(8).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
