A comparison of duck and chicken egg yolk for cryopreservation of stallion sperm.
Abstract: Duck and chicken egg yolk were compared for their protective effects against cold shock during the cryopreservation of stallion sperm in a lactose-EDTA-glycerol cryodiluent. Methods: A completely randomised design was used. Procedure Ejaculates from five stallions (n = 14 ejaculates) were split and diluted to either 20 or 200 x 10(6) sperm/mL in a lactose-EDTA extender containing either duck or chicken egg yolk. The extended semen was then frozen in liquid nitrogen. The percentage of sperm total motility and forward progressive motility were assessed before freezing and at 0 and 1 hr after thawing. Morphology data were also collected at 0 and 1 hr post thaw. Results: Total and forward progressive motility were higher when the sperm were frozen in the presence of duck rather than chicken egg yolk. Furthermore, the total and forward progressive motility and percentage of morphologically normal sperm were higher when frozen at a concentration of 200 than 20 x 10(6)/mL. Conclusions: The results of this study demonstrate that the motility parameters of stallion sperm are improved when the semen is frozen in lactose EDTA extender supplemented with duck egg yolk rather than chicken egg yolk. Moreover, sperm motility and the percentage of morphologically normal sperm were higher after freezing at a concentration of 200 x 10(6)/ml rather than 20 x 10(6)/ml.
Publication Date: 2007-06-06 PubMed ID: 17547636DOI: 10.1111/j.1751-0813.2007.00151.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article entails a comparative study of duck and chicken egg yolk used in cryopreservation, demonstrating that the use of duck egg yolk provided better motility and morphological normalcy to stallion sperm. Also, freezing the semen at a higher concentration was found to yield better results.
Objective of the Research
- The primary aim of this research was to reveal the comparative effects of using duck and chicken egg yolk in the cryopreservation of stallion sperm. The researchers attempted to identify which of these two yolks provides better protection against cold shock during the freezing process.
Research Methodology
- The researchers used a completely randomized design for the experimentation.
- The ejaculates obtained from five different stallions were split and diluted to two separate concentrations: 20 x 10(6) sperm/mL and 200 x 10(6) sperm/mL.
- Each concentration was prepared with a lactose-EDTA extender supplemented with either duck egg yolk or chicken egg yolk.
- The prepared sperm samples were frozen in liquid nitrogen for preservation.
- After freezing and thawing, the sample’s total and forward progressive motility were assessed. The morphology of sperm was evaluated as well at 0 and 1 hour post thawing.
Results of the Study
- It was observed that the samples frozen in the presence of duck egg yolk demonstrated higher total and forward progressive motility compared to samples frozen with chicken egg yolk.
- Apart from this, the concentration of sperm also impacted the results: a concentration of 200 x 10(6) sperm/mL had higher motility and normal sperm percentage compared to the lower concentration.
Conclusions
- Based on the results, the researchers concluded that for cryopreserving stallion sperm, the use of a lactose EDTA extender supplemented with duck egg yolk yields better results in terms of sperm motility and morphology.
- Furthermore, they suggested that freezing sperm at a higher concentration of 200 rather than 20 x 10(6)/mL improves both the motility and percentage of morphologically normal sperm.
Cite This Article
APA
Clulow JR, Maxwell WM, Evans G, Morris LH.
(2007).
A comparison of duck and chicken egg yolk for cryopreservation of stallion sperm.
Aust Vet J, 85(6), 232-235.
https://doi.org/10.1111/j.1751-0813.2007.00151.x Publication
Researcher Affiliations
- Faculty of Veterinary Science, The University of Sydney, NSW 2006. jclulow@vetsci.usyd.edu.au
MeSH Terms
- Animals
- Chickens
- Cryopreservation / methods
- Cryopreservation / veterinary
- Cryoprotective Agents / pharmacology
- Ducks
- Egg Yolk / chemistry
- Horses / physiology
- Male
- Random Allocation
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Count / veterinary
- Sperm Motility / drug effects
- Sperm Motility / physiology
- Spermatozoa / drug effects
- Spermatozoa / physiology
Citations
This article has been cited 2 times.- Swelum AA, Ba-Awadh HA, Olarinre IO, Saadeldin IM, Alowaimer AN. Effects of adding mixed chicken and quail egg yolks to the cryodiluent on the quality of ram semen before and after cryopreservation.. Front Vet Sci 2022;9:1013533.
- Nikitkina E, Musidray A, Krutikova A, Anipchenko P, Plemyashov K, Shiryaev G. Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions.. Animals (Basel) 2020 Oct 4;10(10).
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