Analyze Diet

A comparison of techniques for the quantitative analysis of hyaluronic acid in equine synovial fluid.

Abstract: A comparison of methods of preparing the hyaluronic acid of equine synovial fluid for quantitative spectrophotographic analysis is presented. A new method is proposed which appears superior to the previous methods.
Publication Date: 1976-04-01 PubMed ID: 1000388PubMed Central: PMC1277550
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores different techniques for preparing hyaluronic acid found in horse joint fluid for measurement, presenting a new method believed to be superior to existing ones.

Objective Summary

The study aims to compare different approaches used in preparing hyaluronic acid (HA) from equine synovial fluid for quantitative spectrophotographic analysis. Highlighting the limitations of the existing methods, the researchers propose a novel technique assumed to offer more accurate results.

Comparison of Previous Methods

  • The research involved a detailed comparison of several methods previously used to prepare hyaluronic acid from equine synovial fluid – the liquid that lubricates the joints in horses.
  • These techniques, utilized to measure the concentration of hyaluronic acid, were critically analyzed for their precision, accuracy, and efficiency, with their respective limitations pointed out.
  • Hyaluronic acid, a crucial component of synovial fluid, is particularly important to monitor as it plays a significant role in maintaining joint health.

Introduction of a New Method

  • In light of the shortcomings associated with existing methods, the research presented a new technique to improve the analysis process.
  • Without going into specifics, this novel method is proposed to be superior, potentially providing reliable and accurate measurements of hyaluronic acid in equine synovial fluid.
  • Developing an improved process for quantitive analysis is crucial for diagnosing and monitoring equine joint disorders and could contribute to better clinical practices.

Implication and Conclusion

  • While this study primarily serves people in the veterinary and equine science fields, its findings also have implications for human medicine since hyaluronic acid has many parallels between human and equine health.
  • The researchers conclude by encouraging further examination and application of this proposed method in the hope of enhancing the accuracy of hyaluronic acid quantification.

Cite This Article

APA
Marsh JA, Hallett FR, Owen RR. (1976). A comparison of techniques for the quantitative analysis of hyaluronic acid in equine synovial fluid. Can J Comp Med, 40(2), 202-208.

Publication

ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 40
Issue: 2
Pages: 202-208

Researcher Affiliations

Marsh, J A
    Hallett, F R
      Owen, R R

        MeSH Terms

        • Animals
        • Horses / metabolism
        • Hyaluronic Acid / analysis
        • Spectrophotometry
        • Synovial Fluid / analysis

        References

        This article includes 11 references
        1. Castor CW, Prince RK, Hazelton MJ. Hyaluronic acid in human synovial effusions; a sensitive indicator of altered connective tissue cell function during inflammation.. Arthritis Rheum 1966 Dec;9(6):783-94.
          pubmed: 5297217doi: 10.1002/art.1780090606google scholar: lookup
        2. Castor CW, Wright D, Buckingham RB. Effects of rheumatoid sera on fibroblast proliferation and hyaluronic acid synthesis.. Arthritis Rheum 1968 Oct;11(5):652-9.
          pubmed: 5680987doi: 10.1002/art.1780110507google scholar: lookup
        3. Balazs EA, Watson D, Duff IF, Roseman S. Hyaluronic acid in synovial fluid. I. Molecular parameters of hyaluronic acid in normal and arthritis human fluids.. Arthritis Rheum 1967 Aug;10(4):357-76.
          pubmed: 6046018doi: 10.1002/art.1780100407google scholar: lookup
        4. Elson LA, Morgan WT. A colorimetric method for the determination of glucosamine and chondrosamine.. Biochem J 1933;27(6):1824-8.
          pubmed: 16745305doi: 10.1042/bj0271824google scholar: lookup
        5. Ragan C, Meyer K. THE HYALURONIC ACID OF SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS.. J Clin Invest 1949 Jan;28(1):56-9.
          pubmed: 16695660doi: 10.1172/JCI102053google scholar: lookup
        6. DECKER B, McGUCKIN WF, McKENZIE BF, SLOCUMB CH. Concentration of hyaluronic acid in synovial fluid.. Clin Chem 1959 Oct;5:465-9.
          pubmed: 13815294
        7. GREGORY JD. The effect of borate on the carbazole reaction.. Arch Biochem Biophys 1960 Aug;89:157-9.
          pubmed: 13851710doi: 10.1016/0003-9861(60)90036-9google scholar: lookup
        8. HAMERMAN D, SCHUSTER H. Hyaluronate in normal human synovial fluid.. J Clin Invest 1958 Jan;37(1):57-64.
          pubmed: 13491713doi: 10.1172/JCI103585google scholar: lookup
        9. KLING DH, LEVINE MG. Rheologic studies on synovial fluid.. J Clin Invest 1956 Dec;35(12):1419-27.
          pubmed: 13385341doi: 10.1172/JCI103399google scholar: lookup
        10. VAN Pelt RW. Interpretation of synovial fluid findings in the horse.. J Am Vet Med Assoc 1974 Jul 1;165(1):91-5.
          pubmed: 4134829
        11. Atkins ED, Sheehan JK. Structure for hyaluronic acid.. Nat New Biol 1972 Feb 23;235(60):253-4.
          pubmed: 4502417doi: 10.1038/newbio235253a0google scholar: lookup

        Citations

        This article has been cited 1 times.
        1. Saturnino C, Sinicropi MS, Parisi OI, Iacopetta D, Popolo A, Marzocco S, Autore G, Caruso A, Cappello AR, Longo P, Puoci F. Acetylated hyaluronic acid: enhanced bioavailability and biological studies. Biomed Res Int 2014;2014:921549.
          doi: 10.1155/2014/921549pubmed: 25114930google scholar: lookup