A comparison of the effects of equine luteinizing hormone (eLH), equine growth hormone (eGH) and human recombinant insulin-like growth factor (hrIGF-I) on steroid production in cultured equine Leydig cells during sexual maturation.
Abstract: There are several hormones and local testicular factors involved in the initiation and control of steroidogenesis and spermatogenesis during puberty. GH and its mediator, IGF-I, increase substantially during puberty, and in addition to LH, these growth-promoting hormones can have direct effects upon testicular function. The objective of this work was to investigate the effects of eLH, eGH and hrIGF-I upon Leydig cells derived from testes of colts and stallions representing different stages of development. Testes were obtained from 48 light horse colts and stallions at the time of routine castration, horses were categorized according to age group (prepubertal, pubertal and postpubertal) and a Leydig cell enriched preparation was utilized for cell culture. Cells derived from all 48 horses were treated with doses of eLH, and a subset of 21 horses received doses of eGH and hrIGF-I. Cells were plated at a concentration of 1 x 10(6) cells/ml and incubated for 24 h at 32 degrees C. Production of testosterone and estradiol was measured by validated RIA. Leydig cells from prepubertal colts secreted greater basal amounts of testosterone but lesser basal amounts of estradiol compared with the other age groups (p < 0.001). Pubertal stallions exhibited the greatest relative response to eLH (p < 0.05). Neither eGH nor hrIGF-I elicited a steroidogenic response over baseline concentrations in any of the three age groups.
Publication Date: 2005-08-09 PubMed ID: 16085376DOI: 10.1016/j.anireprosci.2005.06.014Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the effects of certain hormones on steroid production in the Leydig cells (a type of cell found in the testes) of horses at different stages of sexual maturation.
Objective of the Research
This research is aimed at understanding the effects of equine luteinizing hormone (eLH), equine growth hormone (eGH), and human recombinant insulin-like growth factor (hrIGF-I) upon Leydig cells in horses. These Leydig cells are gathered from the testes of colts and stallions of varying developmental stages.
Research Methods
- The testes were collected from 48 light horse colts and stallions during routine castration.
- Horses were divided into different age-based categories namely pre-puberty, puberty, and post-puberty.
- A Leydig cell enriched preparation was used for cell culture.
- Cells derived from these 48 horses were treated with doses of eLH and a subset of 21 horses were additionally treated with eGH and hrIGF-I.
- The cells were maintained at a concentration of 1 x 10(6) cells/ml and incubated for 24 hours at a temperature of 32 degrees Celsius.
Results of the Research
- Leydig cells taken from pre-pubertal colts secreted more testosterone but less estradiol compared to other age groups (this was statistically significant with p < 0.001).
- Pubertal stallions responded the most to eLH (significance was p < 0.05).
- Neither eGH nor hrIGF-I initiated a steroidogenic response above the baseline levels in any of the three age groups. This indicates that these hormones did not noticeably increase the production of steroids in these cells.
This study provides valuable insights into the effects of these hormones on Leydig cells during the different stages of equine sexual maturation. It can aid in better understanding and potentially improving the reproductive health and management of horses.
Cite This Article
APA
Hess MF, Roser JF.
(2005).
A comparison of the effects of equine luteinizing hormone (eLH), equine growth hormone (eGH) and human recombinant insulin-like growth factor (hrIGF-I) on steroid production in cultured equine Leydig cells during sexual maturation.
Anim Reprod Sci, 89(1-4), 7-19.
https://doi.org/10.1016/j.anireprosci.2005.06.014 Publication
Researcher Affiliations
- Department of Animal Science, University of California, Davis, CA 95616, USA.
MeSH Terms
- Animals
- Cells, Cultured
- Estradiol / biosynthesis
- Growth Hormone / pharmacology
- Horses
- Humans
- Insulin-Like Growth Factor I / pharmacology
- Leydig Cells / drug effects
- Leydig Cells / metabolism
- Luteinizing Hormone / pharmacology
- Male
- Sexual Maturation
- Steroids / biosynthesis
- Testosterone / biosynthesis
Citations
This article has been cited 4 times.- Shakeel M, Yoon M. Functions of somatic cells for spermatogenesis in stallions. J Anim Sci Technol 2022 Jul;64(4):654-670.
- Hull KL, Harvey S. Growth hormone and reproduction: a review of endocrine and autocrine/paracrine interactions. Int J Endocrinol 2014;2014:234014.
- Amutha C, Subramanian P. Cadmium alters the reproductive endocrine disruption and enhancement of growth in the early and adult stages of Oreochromis mossambicus. Fish Physiol Biochem 2013 Apr;39(2):351-61.
- Laughlin AM, Welsh TH Jr, Love CC, Varner DD, Parrish AR, Forrest DW, Ing NH. In vitro culture of precision-cut testicular tissue as a novel tool for the study of responses to LH. In Vitro Cell Dev Biol Anim 2010 Jan;46(1):45-53.
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