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Equine veterinary journal2022; 54(6); 1039-1046; doi: 10.1111/evj.13554

A comprehensive and comparative proteomic analysis of horse serum proteins in colitis.

Abstract: Equine colitis is a diarrhoeal disease caused by inflammation of the large bowel and can potentially be life-threatening due to its rapid progression. Pathogenesis is multifactorial and pathophysiology is highly complicated, therefore, reliable diagnostic biomarkers are needed in the veterinary field. Objective: Serum is one of the most commonly used diagnostic tools in equine clinical investigation. To discover diagnostic or prognostic protein markers for colitis in horse serum, comprehensive and comparative proteomic analysis was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: Case-control study. Methods: Serum samples were collected from 36 healthy Thoroughbreds and 12 Thoroughbreds with colitis. Serum from each horse suffering from colitis was collected daily until death or recovery. Collected sera were digested with trypsin. Peptides obtained from serum proteins were measured by Q-Exactive HF Orbitrap mass spectrometer. The identification and quantification of peptides were performed using Proteome Discoverer version 2.2. Results: On day 1 of treatment, eight proteins in the colitis group were upregulated (P < .05, more than a twofold change) compared with the healthy group. Among the eight proteins, biliverdin reductase B was significantly upregulated (P < .05) in the non-survivor group (n = 5) compared with the survivor group (n = 7). On the last day of the treatment, haemoglobin subunit alpha, clusterin, glyceraldehyde-3-phosphate dehydrogenase, lipopolysaccharide-binding protein, and biliverdin reductase B showed significant increases (P < .05) in the non-survivor group. Conclusions: The number of the identified proteins is limited due to the existence of abundant proteins. Conclusions: Measuring the changes of these proteins together may enable a potential prognosis or early diagnosis of horses suffering from colitis.
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Publication Date: 2022-01-18 PubMed ID: 35000251DOI: 10.1111/evj.13554Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated how horse serum proteins change during colitis in Thoroughbred horses in search of potential diagnostic markers for the disease. The method used was a comprehensive and comparative proteomic analysis through the use of liquid chromatography-tandem mass spectrometry.

Overview of the Research

  • The study aimed at identifying potential diagnostic or prognostic protein markers for equine colitis – a potentially fatal inflammation of the large bowel in horses.
  • For this purpose, a comprehensive and comparative proteomic analysis was carried out on serum samples obtained from healthy Thoroughbreds and Thoroughbreds with colitis.
  • The technique used for this analysis was liquid chromatography-tandem mass spectrometry (LC-MS/MS), a powerful tool for identifying and quantifying proteins.

Methodology

  • Serum samples were collected from 36 healthy Thoroughbreds and 12 Thoroughbreds diagnosed with colitis. For those with colitis, daily serum samples were collected until the horse either succumbed to the disease or recovered.
  • The collected serum samples were digested with trypsin to break down proteins into peptides, which are smaller segments that can be more readily measured.
  • The peptides derived from serum proteins were then measured by a Q-Exactive HF Orbitrap mass spectrometer, and data processed using Proteome Discoverer version 2.2 software.

Findings

  • On the first day of treatment, eight proteins were found to be upregulated (increased in expression) in the colitis group compared to the healthy horse group. One of these proteins, biliverdin reductase B, was significantly upregulated in the non-survivor group compared with the survivor group.
  • At the conclusion of the treatment period, five proteins (including biliverdin reductase B) exhibited significant increases in the non-survivor group.

Conclusion of the Study

  • The study concluded that although its findings are limited due to the overwhelming presence of abundant proteins in the samples, the identified modified serum proteins might hold potential for prognosis or early diagnosis of horses suffering from colitis.
  • The research may pave the way for further studies focusing on these proteins as potential diagnostic markers for colitis in horses.

Cite This Article

APA
Minamijima Y, Tozaki T, Kuroda T, Urayama S, Nomura M, Yamamoto K. (2022). A comprehensive and comparative proteomic analysis of horse serum proteins in colitis. Equine Vet J, 54(6), 1039-1046. https://doi.org/10.1111/evj.13554

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 54
Issue: 6
Pages: 1039-1046

Researcher Affiliations

Minamijima, Yohei
  • Laboratory of Racing Chemistry, Utsunomiya, Japan.
  • Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.
Tozaki, Teruaki
  • Laboratory of Racing Chemistry, Utsunomiya, Japan.
Kuroda, Taisuke
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Japan.
Urayama, Shuntaro
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Inashiki, Japan.
Nomura, Motoi
  • Equine Hospital, Horseracing School, Japan Racing Association, Shiroi, Japan.
Yamamoto, Kazuo
  • Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.

MeSH Terms

  • Animals
  • Biomarkers
  • Blood Proteins / analysis
  • Case-Control Studies
  • Chromatography, Liquid / methods
  • Chromatography, Liquid / veterinary
  • Clusterin
  • Colitis / veterinary
  • Hemoglobin Subunits / analysis
  • Horse Diseases / diagnosis
  • Horses
  • Peptides
  • Proteome / analysis
  • Proteomics / methods
  • Tandem Mass Spectrometry / veterinary
  • Trypsin

Grant Funding

  • Laboratory of Racing Chemistry
  • Japan Racing Association

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Citations

This article has been cited 1 times.
  1. Ludwig EK, Hobbs KJ, McKinney-Aguirre CA, Gonzalez LM. Biomarkers of Intestinal Injury in Colic. Animals (Basel) 2023 Jan 7;13(2).
    doi: 10.3390/ani13020227pubmed: 36670767google scholar: lookup
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