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Home / Equine Research Bank / Nucleic Acids Res / A conserved structural element in horse and mouse IGF2 genes...
Nucleic acids research1998; 26(7); 1605-1612; doi: 10.1093/nar/26.7.1605

A conserved structural element in horse and mouse IGF2 genes binds a methylation sensitive factor.

Abstract: The equine IGF2 gene has been cloned and characterised. It spans a 9 kb region, which is substantially less than the corresponding human gene. Three coding exons and three untranslated leader exons, all highly homologous to those in other species, were identified. Downstream of the polyadenylation site in exon 6, a dinucleotide repeat sequence was identified. Three putative promoters (P1-P3) were localised in the 5' region of the gene. RNase protection analysis revealed two active promoters in fetal tissues, P2 and P3, whereas P3 was the only promoter active in adult tissues. This represents a transcriptional pattern different from that in humans or rodents. A novel structural element, an inverted repeat, is predicted in the 3' region of the IGF2 gene. This repeat is conserved between species and located in a region which is differentially methylated in the human and mouse genes and might therefore be involved in the imprinting mechanism. The inverted repeat acquires a stem-loop structure in vitro with a hybrid A/B-DNA conformation in the stem area. Both in horse and mouse, a methylation-sensitive protein binds this structure with a strong requirement for the loop area. Furthermore, the protein might be developmentally regulated.
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Publication Date: 1998-05-30 PubMed ID: 9512529PubMed Central: PMC147450DOI: 10.1093/nar/26.7.1605Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the structure and function of the IGF2 gene in horses, specifically how it interacts with a methylation sensitive factor through a unique structural element. The researchers found distinctions between the IGF2 gene in horses and those in other species, indicating different transcriptional patterns.

Background of the Research

  • The research is centered around the IGF2 gene in horses. The scientists have cloned and examined this gene, finding out that it spans a 9 kb region which is less significant than its human counterpart.
  • The horse IGF2 gene consists of three coding exons and three untranslated leader exons. These exons exhibit a high degree of homology with those of other species.
  • Located downstream of the polyadenylation site in exon 6 is a dinucleotide repeat sequence.
  • Three possible promoters (P1-P3) were located in the 5′ region of the gene.

Analyzing the IGF2 Gene

  • The team used RNase protection analysis, a laboratory method utilized to map the ends of RNA molecules, to identify the active promoters in fetal tissues and adult tissues.
  • Protein coding in fetal tissues was linked to promoters P2 and P3 while only promoter P3 was active in adult tissues, indicating a transcription pattern different from that in humans or rodents.
  • The researchers found a novel structural element, an inverted repeat, in the 3′ region of the IGF2 gene. This repeat appears conserved amongst species and is located in a part of the gene which is differentially methylated in the human and mouse genes.
  • The study postulates that the identified inverted repeat might have a role in the gene imprinting process.

Interacting with a Methylation Sensitive Factor

  • The inverted repeat within the IGF2 gene acquires a stem-loop structure when in vitro and has a hybrid A/B-DNA conformation in its stem area. This conformation is crucial for its interaction with a methylation-sensitive protein.
  • Both in the horse and mouse, a methylation-sensitive protein binds with the stem-loop structure, with a strong requirement for the loop area. This suggests a functional significance for this part of the gene.
  • Additionally, the researchers propose that the production of this methylation-sensitive protein might be affected by a developmental regulation mechanism.

Cite This Article

APA
Otte K, Choudhury D, Charalambous M, Engström W, Rozell B. (1998). A conserved structural element in horse and mouse IGF2 genes binds a methylation sensitive factor. Nucleic Acids Res, 26(7), 1605-1612. https://doi.org/10.1093/nar/26.7.1605

Publication

Nucleic acids research
ISSN: 0305-1048
NlmUniqueID: 0411011
Country: England
Language: English
Volume: 26
Issue: 7
Pages: 1605-1612

Researcher Affiliations

Otte, K
  • Department of Pathology, Swedish University of Agricultural Sciences, PO Box 7028, 75007 Uppsala, Sweden. ko213@mole.biol.cam.ac.uk
Choudhury, D
    Charalambous, M
      Engström, W
        Rozell, B

          MeSH Terms

          • Animals
          • Base Sequence
          • Conserved Sequence
          • DNA / chemistry
          • DNA / genetics
          • DNA / metabolism
          • DNA Methylation
          • Exons
          • Fetus
          • Genomic Imprinting
          • Genomic Library
          • Horses / genetics
          • Humans
          • Insulin-Like Growth Factor II / biosynthesis
          • Insulin-Like Growth Factor II / genetics
          • Kidney / embryology
          • Kidney / metabolism
          • Liver / embryology
          • Liver / metabolism
          • Mice / genetics
          • Molecular Sequence Data
          • Nucleic Acid Conformation
          • Poly A
          • Promoter Regions, Genetic
          • Protein Sorting Signals / biosynthesis
          • Protein Sorting Signals / genetics
          • Repetitive Sequences, Nucleic Acid

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          Citations

          This article has been cited 5 times.
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