A new surface marker on equine peripheral blood lymphocytes. II. Characterization and separation of purified blood lymphocytes with receptors for Helix pomatia A hemagglutinin (HP).
Abstract: In a preceding report we have shown that the lectin Helix pomatia A hemagglutinin (HP) binds to two subpopulations of neuraminidase-treated equine peripheral blood lymphocytes (PBL), constituting about 20% and 75% of PBL, respectively. The aim of the present study was to further characterize these HP+ cells in regard to other surface markers such as receptors for guinea pig erythrocytes (GPR+ cells), membrane-bound immunoglobulins (sIg+ cells), receptors for activated complement (C3R+ cells) and receptors for IgG (Fc alpha R+ cells). This was done by double marker analysis and by lymphocyte fractionation either on columns charged with HP coupled to Sepharose beads or by rosetting with guinea pig erythrocytes. The fractions were also analysed for their proliferative response in the mixed lymphocyte tumor cell interaction (MLTC) assay and to the mitogens leucoagglutinin (La) and concanavalin A (Con A). The results revealed that the majority of GPR+ cells also expressed high avidity receptors for HP, as defined by means of direct immunofluorescence. These cells constituted a subpopulation of GPR+/HP+ cells T cells comprising approximately 20% of PBL. In contrast, about 75% of the HP+ cells in indirect immunofluorescence were GPR-. The fractionation experiments showed that HP+ and GPR+ cells were probably not B cells since they were sIg-. The C3R+ and Fc alpha R+ lymphocytes were heterogeneous in regard to HP receptors but the majority of these cells was also found in the fractions depleted of HP+ and GPR+ lymphocytes. The fractions eluted from HP columns gave a strong proliferative response in MLTC, whereas fractions depleted of HP+ cells responded poorly. However, in contrast to the GPR+-depleted fractions, those enriched in GPR+ lymphocytes responded poorly to the T cell mitogens La and Con A. The mitogenic response of the HP-column fractions to La and to Con A was variable. The results are discussed in relation to HP being a surface marker for a heterogeneous population of equine T cells.
Publication Date: 1985-01-01 PubMed ID: 3976170DOI: 10.1016/0165-2427(85)90109-6Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers have been studying a subset of blood cells in horses that have a receptor for a certain substance known as Helix pomatia A hemagglutinin (HP). They discovered that a large proportion of these cells also have receptors for other substances and they also examined how these cells respond to certain triggers.
Highlighted Research Processes
- The study aims to characterize cells in horse blood that have a receptor for HP, which was previously found to bind with two sub-types of cells known as peripheral blood lymphocytes (PBL).
- The cells were also evaluated for a variety of other surface markers, including receptors for guinea pig red blood cells, membrane-bound immunoglobulins, receptors for a part of the immune system called activated complement, and receptors for a type of antibody called IgG.
- Double marker analysis and cell fractionation – techniques used to distinguish different cell types – were used for characterization.
- The researchers also assessed the cells’ reaction to certain triggers, such as those used in an assay to measure cell growth and response to mitogens – substances that trigger cell division.
Discoveries and Conclusions
- Cells with receptors for guinea pig red blood cells largely also had high-activity receptors for HP.
- However, the majority of HP-receptive cells did not have receptors for guinea pig red blood cells.
- HP-receptive cells and those receptive to guinea pig red blood cells were unlikely to be B cells, another category of immune cells, as they did not have membrane-bound immunoglobulins.
- Cells with activated complement and IgG receptors were mixed in terms of HP receptor presence, however, most were found in fractions that did not contain HP or guinea pig red blood cell receptors.
- The HP-receptive cells demonstrated a strong response in the mixed lymphocyte tumor cell interaction assay, indicating high rates of growth and division.
- However, cells with guinea pig red blood cell receptors, a type of T cell, responded poorly to the mitogens leucoagglutinin and concanavalin A.
- These findings suggest that HP may be a surface marker for a range of T cells in horses.
Cite This Article
APA
Broström H, Hellström U, Ziverts I, Obel N, Perlmann P.
(1985).
A new surface marker on equine peripheral blood lymphocytes. II. Characterization and separation of purified blood lymphocytes with receptors for Helix pomatia A hemagglutinin (HP).
Vet Immunol Immunopathol, 8(1-2), 47-61.
https://doi.org/10.1016/0165-2427(85)90109-6 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cell Separation
- Female
- Horses / blood
- Horses / immunology
- Lectins / immunology
- Lymphocyte Activation
- Lymphocytes / immunology
- Male
- Neuraminidase
- Receptors, Antigen, B-Cell
- Receptors, Complement
- Receptors, Mitogen
- Rosette Formation
Citations
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