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Journal of clinical microbiology1991; 29(9); 2043-2046; doi: 10.1128/jcm.29.9.2043-2046.1991

A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23.

Abstract: Equine rotavirus FI23 was shown to be prototypic of a novel G serotype, provisionally G14, by cross-neutralization and VP7 sequence determination. Although distinct, there are as few as six differing amino acid residues (92, 94, 96, 146, 147, and 221) in the VP7 antigenic regions of FI23 and G3 rotaviruses.
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Publication Date: 1991-09-01 PubMed ID: 1663521PubMed Central: PMC270257DOI: 10.1128/jcm.29.9.2043-2046.1991Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses the identification of a new group A rotavirus serotype, provisionally named G14, using the equine isolate FI23. The study performed cross-neutralization and VP7 sequence DNA determination to establish the unique characteristics of this strain, which only has six distinguishing amino acids from the G3 serotypes.

Study Objective and Methodology

  • The objective of this study was to characterize a newly identified group A rotavirus serotype. The researchers used an equine rotavirus strain, FI23, for this study. They aimed to define the unique properties of this novel strain in relation to other known serotypes.
  • In order to achieve this objective, the authors used two main methods, cross-neutralization and VP7 sequence determination. Cross-neutralization is a test which reveals how the virus interacts with antibodies, giving insight into whether it’s a new strain or a variation of an existing one. The VP7 sequence determination, on the other hand, is a genomic study of the virus’s outer protein layer which helps in identifying and differentiating between various rotavirus serotypes.

Key Findings

  • The results from the cross-neutralization test and VP7 sequence determination suggest that the equine rotavirus FI23 is a novel serotype, distinct from existing types. The authors propose the designation G14 to acknowledge this finding.
  • While the G14 strain exhibits distinct properties, it admittedly shares a substantial genetic similarity with the G3 rotaviruses. Particularly, there are only six differing amino acid residues in the VP7 antigenic regions of FI23 and G3 rotaviruses. These residues are 92, 94, 96, 146, 147, and 221, providing the basis for genomic difference between G14 and G3 serotypes.

Importance and Implications of the Study

  • Identification of new group A rotavirus serotypes, such as G14, is crucial for understanding the biology of the virus, and in the development of effective vaccines.
  • Although G14 shares genetic similarities with other serotypes, the presence of the six differing amino acid residues confirms that it is a distinct form. This genetic variance can influence how the virus interacts with the immune system and informs further development of medical interventions.

Cite This Article

APA
Browning GF, Fitzgerald TA, Chalmers RM, Snodgrass DR. (1991). A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol, 29(9), 2043-2046. https://doi.org/10.1128/jcm.29.9.2043-2046.1991

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 29
Issue: 9
Pages: 2043-2046

Researcher Affiliations

Browning, G F
  • Moredun Research Institute, Edinburgh, Scotland.
Fitzgerald, T A
    Chalmers, R M
      Snodgrass, D R

        MeSH Terms

        • Amino Acid Sequence
        • Animals
        • Antigens, Viral / genetics
        • Base Sequence
        • Capsid / genetics
        • Capsid / immunology
        • Capsid Proteins
        • DNA, Viral / genetics
        • Horses
        • Molecular Sequence Data
        • Rotavirus / classification
        • Rotavirus / genetics
        • Rotavirus / isolation & purification
        • Serotyping

        References

        This article includes 32 references
        1. Mackow ER, Shaw RD, Matsui SM, Vo PT, Benfield DA, Greenberg HB. Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus.. Virology 1988 Aug;165(2):511-7.
          pubmed: 2457279doi: 10.1016/0042-6822(88)90595-8google scholar: lookup
        2. Nishikawa K, Hoshino Y, Taniguchi K, Green KY, Greenberg HB, Kapikian AZ, Chanock RM, Gorziglia M. Rotavirus VP7 neutralization epitopes of serotype 3 strains.. Virology 1989 Aug;171(2):503-15.
          pubmed: 2474892doi: 10.1016/0042-6822(89)90620-xgoogle scholar: lookup
        3. Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachman P, Offit PA. Rotavirus isolate WI61 representing a presumptive new human serotype.. J Clin Microbiol 1987 Sep;25(9):1757-62.
          pubmed: 2443534doi: 10.1128/jcm.25.9.1757-1762.1987google scholar: lookup
        4. Hoshino Y, Gorziglia M, Valdesuso J, Askaa J, Glass RI, Kapikian AZ. An equine rotavirus (FI-14 strain) which bears both subgroup I and subgroup II specificities on its VP6.. Virology 1987 Apr;157(2):488-96.
          pubmed: 2435059doi: 10.1016/0042-6822(87)90291-1google scholar: lookup
        5. Dyall-Smith ML, Lazdins I, Tregear GW, Holmes IH. Location of the major antigenic sites involved in rotavirus serotype-specific neutralization.. Proc Natl Acad Sci U S A 1986 May;83(10):3465-8.
          pubmed: 2422651doi: 10.1073/pnas.83.10.3465google scholar: lookup
        6. Gunn PR, Sato F, Powell KF, Bellamy AR, Napier JR, Harding DR, Hancock WS, Siegman LJ, Both GW. Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis.. J Virol 1985 Jun;54(3):791-7.
          pubmed: 2582147doi: 10.1128/JVI.54.3.791-797.1985google scholar: lookup
        7. Mason BB, Dheer SK, Hsiao CL, Zandle G, Kostek B, Rosanoff EI, Hung PP, Davis AR. Sequence of the serotype-specific glycoprotein of the human rotavirus Wa strain and comparison with other human rotavirus serotypes.. Virus Res 1985 Jun;2(4):291-9.
          pubmed: 2412362doi: 10.1016/0168-1702(85)90026-7google scholar: lookup
        8. Huang J, Nagesha HS, Dyall-Smith ML, Holmes IH. Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses.. Arch Virol 1989;109(3-4):173-83.
          pubmed: 2558633doi: 10.1007/BF01311079google scholar: lookup
        9. Green KY, Hoshino Y, Ikegami N. Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes.. Virology 1989 Feb;168(2):429-33.
          pubmed: 2536991doi: 10.1016/0042-6822(89)90289-4google scholar: lookup
        10. Reddy DA, Greenberg HB, Bellamy AR. Rotavirus serotype IV: nucleotide sequence of genomic segment nine of the St Thomas 3 strain.. Nucleic Acids Res 1989 Jan 11;17(1):449.
          pubmed: 2536151doi: 10.1093/nar/17.1.449google scholar: lookup
        11. Snodgrass DR, Fitzgerald T, Campbell I, Scott FM, Browning GF, Miller DL, Herring AJ, Greenberg HB. Rotavirus serotypes 6 and 10 predominate in cattle.. J Clin Microbiol 1990 Mar;28(3):504-7.
          pubmed: 2157736doi: 10.1128/jcm.28.3.504-507.1990google scholar: lookup
        12. Hoshino Y, Wyatt RG, Greenberg HB, Flores J, Kapikian AZ. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.. J Infect Dis 1984 May;149(5):694-702.
          pubmed: 6202807doi: 10.1093/infdis/149.5.694google scholar: lookup
        13. Elleman TC, Hoyne PA, Dyall-Smith ML, Holmes IH, Azad AA. Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus.. Nucleic Acids Res 1983 Jul 25;11(14):4689-701.
          pubmed: 6308556doi: 10.1093/nar/11.14.4689google scholar: lookup
        14. Hoshino Y, Wyatt RG, Greenberg HB, Kalica AR, Flores J, Kapikian AZ. Isolation, propagation, and characterization of a second equine rotavirus serotype.. Infect Immun 1983 Sep;41(3):1031-7.
          pubmed: 6309657doi: 10.1128/iai.41.3.1031-1037.1983google scholar: lookup
        15. Hoshino Y, Wyatt RG, Greenberg HB, Kalica AR, Flores J, Kapikian AZ. Isolation and characterization of an equine rotavirus.. J Clin Microbiol 1983 Sep;18(3):585-91.
          pubmed: 6313746doi: 10.1128/jcm.18.3.585-591.1983google scholar: lookup
        16. Gillespie J, Kalica A, Conner M, Schiff E, Barr M, Holmes D, Frey M. The isolation, propagation and characterization of tissue-cultured equine rotaviruses.. Vet Microbiol 1984 Feb;9(1):1-14.
          pubmed: 6326375doi: 10.1016/0378-1135(84)90074-9google scholar: lookup
        17. Richardson MA, Iwamoto A, Ikegami N, Nomoto A, Furuichi Y. Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotavirus: comparison with the homologous genes from simian SA11 and UK bovine rotaviruses.. J Virol 1984 Sep;51(3):860-2.
          pubmed: 6088808doi: 10.1128/JVI.51.3.860-862.1984google scholar: lookup
        18. Flores J, Myslinski J, Kalica AR, Greenberg HB, Wyatt RG, Kapikian AZ, Chanock RM. In vitro transcription of two human rotaviruses.. J Virol 1982 Sep;43(3):1032-7.
          pubmed: 6292446doi: 10.1128/JVI.43.3.1032-1037.1982google scholar: lookup
        19. ARCHETTI I, HORSFALL FL Jr. Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum.. J Exp Med 1950 Nov 1;92(5):441-62.
          pubmed: 14778924doi: 10.1084/jem.92.5.441google scholar: lookup
        20. Xu L, Harbour D, McCrae MA. Sequence of the gene encoding the major neutralization antigen (VP7) of serotype 10 rotavirus.. J Gen Virol 1991 Jan;72 ( Pt 1):177-80.
          pubmed: 1703560doi: 10.1099/0022-1317-72-1-177google scholar: lookup
        21. Browning GF, Chalmers RM, Fitzgerald TA, Snodgrass DR. Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype.. J Gen Virol 1991 May;72 ( Pt 5):1059-64.
          pubmed: 1851806doi: 10.1099/0022-1317-72-5-1059google scholar: lookup
        22. Ohta C, Hoshi A, Goto H, Tsunoda N, Tagami M, Akita H. Epizootiological and virological studies of foal diarrhea associated with serotype 3 rotavirus.. Nihon Juigaku Zasshi 1990 Oct;52(5):1049-56.
          pubmed: 2177800doi: 10.1292/jvms1939.52.1049google scholar: lookup
        23. Taniguchi K, Urasawa T, Kobayashi N, Gorziglia M, Urasawa S. Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity.. J Virol 1990 Nov;64(11):5640-4.
          pubmed: 2170690doi: 10.1128/JVI.64.11.5640-5644.1990google scholar: lookup
        24. Hoshino Y, Saif LJ, Sereno MM, Chanock RM, Kapikian AZ. Infection immunity of piglets to either VP3 or VP7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen.. J Virol 1988 Mar;62(3):744-8.
          pubmed: 2828669doi: 10.1128/JVI.62.3.744-748.1988google scholar: lookup
        25. Green KY, Midthun K, Gorziglia M, Hoshino Y, Kapikian AZ, Chanock RM, Flores J. Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes.. Virology 1987 Nov;161(1):153-9.
          pubmed: 2823458doi: 10.1016/0042-6822(87)90181-4google scholar: lookup
        26. Ruiz AM, López IV, López S, Espejo RT, Arias CF. Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype.. J Virol 1988 Nov;62(11):4331-6.
          pubmed: 2845146doi: 10.1128/JVI.62.11.4331-4336.1988google scholar: lookup
        27. Gorziglia M, Hoshino Y, Buckler-White A, Blumentals I, Glass R, Flores J, Kapikian AZ, Chanock RM. Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection.. Proc Natl Acad Sci U S A 1986 Sep;83(18):7039-43.
          pubmed: 3018754doi: 10.1073/pnas.83.18.7039google scholar: lookup
        28. Gorziglia M, Aguirre Y, Hoshino Y, Esparza J, Blumentals I, Askaa J, Thompson M, Glass RI, Kapikian AZ, Chanock RM. VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence.. J Gen Virol 1986 Nov;67 ( Pt 11):2445-54.
          pubmed: 3023532doi: 10.1099/0022-1317-67-11-2445google scholar: lookup
        29. Offit PA, Clark HF, Blavat G, Greenberg HB. Reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype.. J Virol 1986 Nov;60(2):491-6.
          pubmed: 3021983doi: 10.1128/JVI.60.2.491-496.1986google scholar: lookup
        30. Hoshino Y, Sereno MM, Midthun K, Flores J, Kapikian AZ, Chanock RM. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity.. Proc Natl Acad Sci U S A 1985 Dec;82(24):8701-4.
          pubmed: 3001716doi: 10.1073/pnas.82.24.8701google scholar: lookup
        31. Matsuno S, Hasegawa A, Mukoyama A, Inouye S. A candidate for a new serotype of human rotavirus.. J Virol 1985 May;54(2):623-4.
          pubmed: 2985825doi: 10.1128/JVI.54.2.623-624.1985google scholar: lookup
        32. Taniguchi K, Hoshino Y, Nishikawa K, Green KY, Maloy WL, Morita Y, Urasawa S, Kapikian AZ, Chanock RM, Gorziglia M. Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies.. J Virol 1988 Jun;62(6):1870-4.
          pubmed: 2452893doi: 10.1128/JVI.62.6.1870-1874.1988google scholar: lookup

        Citations

        This article has been cited 48 times.
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