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Home / Equine Research Bank / J Clin Microbiol / A novel group A rotavirus G serotype: serological and genomi...
Journal of clinical microbiology1991; 29(9); 2043-2046; doi: 10.1128/jcm.29.9.2043-2046.1991

A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23.

Abstract: Equine rotavirus FI23 was shown to be prototypic of a novel G serotype, provisionally G14, by cross-neutralization and VP7 sequence determination. Although distinct, there are as few as six differing amino acid residues (92, 94, 96, 146, 147, and 221) in the VP7 antigenic regions of FI23 and G3 rotaviruses.
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Publication Date: 1991-09-01 PubMed ID: 1663521PubMed Central: PMC270257DOI: 10.1128/jcm.29.9.2043-2046.1991Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses the identification of a new group A rotavirus serotype, provisionally named G14, using the equine isolate FI23. The study performed cross-neutralization and VP7 sequence DNA determination to establish the unique characteristics of this strain, which only has six distinguishing amino acids from the G3 serotypes.

Study Objective and Methodology

  • The objective of this study was to characterize a newly identified group A rotavirus serotype. The researchers used an equine rotavirus strain, FI23, for this study. They aimed to define the unique properties of this novel strain in relation to other known serotypes.
  • In order to achieve this objective, the authors used two main methods, cross-neutralization and VP7 sequence determination. Cross-neutralization is a test which reveals how the virus interacts with antibodies, giving insight into whether it’s a new strain or a variation of an existing one. The VP7 sequence determination, on the other hand, is a genomic study of the virus’s outer protein layer which helps in identifying and differentiating between various rotavirus serotypes.

Key Findings

  • The results from the cross-neutralization test and VP7 sequence determination suggest that the equine rotavirus FI23 is a novel serotype, distinct from existing types. The authors propose the designation G14 to acknowledge this finding.
  • While the G14 strain exhibits distinct properties, it admittedly shares a substantial genetic similarity with the G3 rotaviruses. Particularly, there are only six differing amino acid residues in the VP7 antigenic regions of FI23 and G3 rotaviruses. These residues are 92, 94, 96, 146, 147, and 221, providing the basis for genomic difference between G14 and G3 serotypes.

Importance and Implications of the Study

  • Identification of new group A rotavirus serotypes, such as G14, is crucial for understanding the biology of the virus, and in the development of effective vaccines.
  • Although G14 shares genetic similarities with other serotypes, the presence of the six differing amino acid residues confirms that it is a distinct form. This genetic variance can influence how the virus interacts with the immune system and informs further development of medical interventions.

Cite This Article

APA
Browning GF, Fitzgerald TA, Chalmers RM, Snodgrass DR. (1991). A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol, 29(9), 2043-2046. https://doi.org/10.1128/jcm.29.9.2043-2046.1991

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 29
Issue: 9
Pages: 2043-2046

Researcher Affiliations

Browning, G F
  • Moredun Research Institute, Edinburgh, Scotland.
Fitzgerald, T A
    Chalmers, R M
      Snodgrass, D R

        MeSH Terms

        • Amino Acid Sequence
        • Animals
        • Antigens, Viral / genetics
        • Base Sequence
        • Capsid / genetics
        • Capsid / immunology
        • Capsid Proteins
        • DNA, Viral / genetics
        • Horses
        • Molecular Sequence Data
        • Rotavirus / classification
        • Rotavirus / genetics
        • Rotavirus / isolation & purification
        • Serotyping

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        Citations

        This article has been cited 48 times.
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