A novel surface plasmon resonance biosensor based on the PDA-AgNPs-PDA-Au film sensing platform for horse IgG detection.
Abstract: Herein we report a novel polydopamine-silver nanoparticle-polydopamine-gold (PDA-AgNPs-PDA-Au) film based surface plasmon resonance (SPR) biosensor for horse IgG detection. The PDA-AgNPs-PDA-Au film sensing platform was built on Au-film via layer-by-layer self-assembly. Ag ion was reduced in situ to AgNPs in presence of PDA. The top PDA layer can prevent AgNPs from being oxidized and connect with antibody via Schiff alkali reaction directly. The morphology and thickness of the modified gold film were characterized using scanning electron microscope and Talystep. Experimental results show that the PDA-AgNPs-PDA-Au film sensing platform is stable, regenerative and sensitive for horse IgG detection. The detection limit of horse IgG obtained with the present biosensor is 0.625μgmL, which is 2-fold and 4-fold lower than that obtained with biosensor based on PDA modified Au film and conventional biosensor based on MPA, respectively. Furthermore, when challenged to real serum samples, our sensor exhibited excellent specificity to horse IgG, suggesting its potential for industrial application.
Copyright © 2017 Elsevier B.V. All rights reserved.
Publication Date: 2017-10-12 PubMed ID: 29054067DOI: 10.1016/j.saa.2017.10.039Google Scholar: Lookup
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Summary
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The research is about a newly developed biosensor built on a polydopamine-silver nanoparticle-polydopamine-gold (PDA-AgNPs-PDA-Au) film, aimed at detecting horse immunoglobulin G (IgG) with enhanced precision and stability.
Creation of the PDA-AgNPs-PDA-Au Film Sensing Platform
- The research centered around the development of a surface plasmon resonance (SPR) biosensor specifically designed for the detection of horse IgG, an antibody that plays a crucial role in immune responses.
- This biosensor was constructed by layering polydopamine (PDA), silver nanoparticles (AgNPs), and gold (Au) on an Au film using a self-assembly technique.
- Silver ions were in-situ converted into AgNPs in the presence of PDA, forming the second layer on the Au film. The top layer, again PDA, served dual functions – it protected the AgNPs from oxidation and helped bind with the antibody through Schiff alkali reaction.
Characterization and Performance of the Sensor
- The Meticulous characterization using a scanning electron microscope and Talystep helped understand the morphology and thickness of the modified gold film.
- During the test trials, the newly developed biosensor demonstrated greater stability, sensitivity, and regenerative properties, significantly improving upon the earlier models.
- The detection limit of horse IgG with the current biosensor reached 0.625μgmL, showing remarkable performance that was two and four times better than the PDA-modified Au film biosensor and the conventional biosensor based on MPA (Mercaptopropionic acid), respectively.
Potential for Real-world Application
- The study also tested the biosensor using real serum samples to determine its practicality and applicability. It exhibited impressive specificity for horse IgG, indicating its immense potential for industrial use.
- Overall, the research brings to light an innovative, efficient biosensor making strides in detecting horse IgG, which could be instrumental in creating effective immunization strategies or understanding immune responses.
Cite This Article
APA
Wang N, Zhang D, Deng X, Sun Y, Wang X, Ma P, Song D.
(2017).
A novel surface plasmon resonance biosensor based on the PDA-AgNPs-PDA-Au film sensing platform for horse IgG detection.
Spectrochim Acta A Mol Biomol Spectrosc, 191, 290-295.
https://doi.org/10.1016/j.saa.2017.10.039 Publication
Researcher Affiliations
- Department of Medical Science & Education, Jilin Province People's Hospital, Changchun 130021, PR China.
- College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
- College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
- College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
- College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
- College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: mapinyi@jlu.edu.cn.
- College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: songdq@jlu.edu.cn.
MeSH Terms
- Animals
- Biosensing Techniques / methods
- Horses
- Humans
- Immunoglobulin G / analysis
- Indoles / chemistry
- Kinetics
- Metal Nanoparticles / chemistry
- Metal Nanoparticles / ultrastructure
- Polymers / chemistry
- Protein Stability
- Silver / chemistry
- Surface Plasmon Resonance
Citations
This article has been cited 8 times.- Ntemafack A, Dzelamonyuy A, Nchinda G, Bopda Waffo A. Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing. Viruses 2023 Jun 22;15(7).
- Azam T, Bukhari SH, Liaqat U, Miran W. Emerging Methods in Biosensing of Immunoglobin G-A Review. Sensors (Basel) 2023 Jan 6;23(2).
- Jing J, Liu K, Jiang J, Xu T, Wang S, Ma J, Zhang Z, Zhang W, Liu T. Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit. Nanomaterials (Basel) 2021 Aug 22;11(8).
- Liu K, Zhang J, Jiang J, Xu T, Wang S, Chang P, Zhang Z, Ma J, Liu T. Multi-layer optical fiber surface plasmon resonance biosensor based on a sandwich structure of polydopamine-MoSe(2)@Au nanoparticles-polydopamine. Biomed Opt Express 2020 Dec 1;11(12):6840-6851.
- Huang Q, Wu W, Ai K, Liu J. Highly Sensitive Polydiacetylene Ensembles for Biosensing and Bioimaging. Front Chem 2020;8:565782.
- Liu Z, He J, He S. Characterization and Sensing of Inert Gases with a High-Resolution SPR Sensor. Sensors (Basel) 2020 Jun 10;20(11).
- Wang Y, Xu H, Zhuang Z, He J, Peng W, Zhang X, Chen H, Zhou L. Preparation of Highly Active Mg-Al-Li-B Alloys via High-Temperature Sintering. Materials (Basel) 2026 Jan 6;19(2).
- Omar NAS, Fen YW, Abdullah J, Mustapha Kamil Y, Daniyal WMEMM, Sadrolhosseini AR, Mahdi MA. Sensitive Detection of Dengue Virus Type 2 E-Proteins Signals Using Self-Assembled Monolayers/Reduced Graphene Oxide-PAMAM Dendrimer Thin Film-SPR Optical Sensor. Sci Rep 2020 Feb 11;10(1):2374.
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