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Acta veterinaria Scandinavica1990; 31(2); 187-192; doi: 10.1186/BF03547561

A preliminary study of uterine derived polymorphonuclear cell function in mares with chronic uterine infections.

Abstract: From 6 mares with chronic uterine infection, polymorphonuclear neutrophils (PMNs) were obtained from the uterus. In order to recover an adequate number of viable PMNs, 0.1% oyster glycogen was infused into the uterus as a mild irritant 12 h prior to the uterine flushing. Phagocytosis and chemotaxis of the uterine derived PMNs were determined. The supernatant from the uterine flushing was compared with autologous serum for its capacity as an opsonin and chemoattractant. There was a significant increase of both phagocytosis and chemotaxis when autologous serum was used compared with the supernatant from the uterine flushing. This study indicates that autologous serum has a greater opsonizing capacity than uterine secretion from mares with chronic uterine infection. Since all mares conceived following this study, the use of oyster glycogen was not considered to have deleterious effect on the uterine mucosa. Från 6 ston med kronisk livmoderinfektion samlades neutrofila granulocyter från livmodern. För att få ett tillråckligt antal levande neutrofila granulocyter infunderades 0.1 %-ig oyster glycogen i livmodern, som ett svagt irritationsmedel 12 timmar före livmodersköljningen. Fagocytos och kemotaxis bestämdes hos neutrofila granulocyter från livmodern. Supernatanten från livmodersköljningen jämfördes med autologt serum avseende opsoniserande och kemotaktiska fbrmåga. Det var en signifikant ökning av fagocytos och kemotaxis när autologt serum användes jämfört med supernatanten från livmodersköljningen. Denna studie indikerar att serum har en positiv effekt på fagocytos och kemotaxis hos neutrofila granulocyter från livmodern i jämförelse med livmodersekret från ston med kronisk livmoderinfektion. Då alla ston blev dräktiga efter detta experiment ansågs användningen av oyster glykogen inte ha någon skadlig effekt på livmoderns slemhinna.
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Publication Date: 1990-01-01 PubMed ID: 2260512PubMed Central: PMC8133245DOI: 10.1186/BF03547561Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study set out to investigate the function of specific immune cells obtained from the uterus of mares with chronic uterine infections. It concluded that a type of serum from the same animal (autologous serum) has more capacity to boost the immune response than fluids from the infected uterus, and that infusing the uterus with oyster glycogen (a mild irritant) does not harm the uterine lining.

Objective of the Research

  • The primary aim of the research is to understand the function of polymorphonuclear neutrophils (PMNs), a type of white blood cell, in mares that have chronic uterine infections. The study also compares the effectiveness of autologous serum and uterine fluid (supernatant) in promoting the immune system’s response.

Research Methodology

  • The researchers extracted PMNs from the uterus of six mares with a chronic uterine infection.
  • To gather an adequate number of PMNs, 0.1% oyster glycogen was administered into the uterus as a light irritant 12 hours before the uterine flushing (the process of extracting the PMNs).
  • The study then ascertained the phagocytosis (the process of cells ingesting harmful particles, bacteria, or dead cells) and chemotaxis (movement of organisms in response to chemicals) of the uterine-derived PMNs.
  • The supernatant from the uterine flushing was compared with autologous serum to see which had a better capacity as an opsonin (substances that make bacteria more susceptible to phagocytosis) and chemoattractant (agents that induce chemotaxis).

Results and Conclusion

  • The research found a significant increase in both phagocytosis and chemotaxis when autologous serum was used instead of the supernatant from the uterine flushing. This suggests that autologous serum has superior opsonizing capacity than uterine secretions from mares with chronic uterine infections.
  • The research also revealed that the infusion of oyster glycogen did not have a detrimental effect on the uterine mucosa (lining), as all the mares conceived after the study.

Cite This Article

APA
Troedsson M, Concha C, Einarsson S, Holmberg O. (1990). A preliminary study of uterine derived polymorphonuclear cell function in mares with chronic uterine infections. Acta Vet Scand, 31(2), 187-192. https://doi.org/10.1186/BF03547561

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 31
Issue: 2
Pages: 187-192

Researcher Affiliations

Troedsson, M
  • Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala.
Concha, C
    Einarsson, S
      Holmberg, O

        MeSH Terms

        • Animals
        • Chronic Disease
        • Endometritis / immunology
        • Endometritis / veterinary
        • Female
        • Horse Diseases / immunology
        • Horses
        • Neutrophils / immunology
        • Uterus / immunology

        References

        This article includes 14 references
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