A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.
Abstract: An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1--1.5 g/20 l). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98--100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.
Publication Date: 1978-12-15 PubMed ID: 729750DOI: 10.1007/BF02034734Google Scholar: Lookup
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- Journal Article
Summary
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The research paper describes a quicker method for obtaining pure eosinophilic leukocytes (a type of white blood cell) from horse blood, producing high yield and maintaining their viability and purity.
Introduction and Objectives
- The research paper presents a simplified and accelerated method for isolating eosinophilic leukocytes from horse blood.
- The objective is to increase the viability and purity of the resulting leukocytes, thereby enhancing the yield (1-1.5g per 20 liters).
Methodology
- The researchers have created an improved process for isolating eosinophilic leukocytes, providing a more efficient alternative to previous, more time-consuming methods.
- Though the exact procedure isn’t detailed in the abstract, it’s reasonable to infer that it involves some form of specialized filtration or centrifugation to separate the eosinophils from the rest of the blood components.
Verification and Results
- The purity and viability of the resulting leukocytes were verified using light and electron microscopy, giving a visual confirmation of their condition after the isolation process.
- The researchers also used a trypan blue exclusion test, a commonly used method to determine the cell viability by showing the number of healthy (trypan blue excluding) cells present. Thus, they ensured not only the purity but also the survival and functionality of the cells after isolation.
- The leukocytes they isolated demonstrated a remarkable 98-100% purity, setting a compelling standard for the effectiveness of the method.
Implications and Further Applications
- Importantly, the researchers showed that these isolated eosinophils were able to phagocytize immune complexes, meaning they remained functional and could still absorb and break down pathogens – a crucial aspect of their role within the immune system.
- This research potentially opens up new, more efficient ways of studying eosinophilic leukocytes and can be quite essential for future research and applications, particularly within immunology and veterinary sciences.
Cite This Article
APA
Jörg A, Portmann P, Fellay G, Dreyer JL, Meyer J.
(1978).
A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.
Experientia, 34(12), 1654-1656.
https://doi.org/10.1007/BF02034734 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cell Separation / methods
- Centrifugation
- Eosinophils / cytology
- Horses
References
This article includes 4 references
- West BC, Gelb NA, Rosenthal AS. Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils.. Am J Pathol 1975 Dec;81(3):575-88.
- ARCHER RK. Studies with eosinophil leucocytes isolated from the blood of the horse.. Br J Haematol 1960 Jul;6:229-41.
- Olsson I, Venge P, Spitznagel JK, Lehrer RI. Arginine-rich cationic proteins of human eosinophil granules: comparison of the constituents of eosinophilic and neutrophilic leukocytes.. Lab Invest 1977 May;36(5):493-500.
- Gessner TP, Himmelhoch R, Shelton E. Partial characterization of the protein components of eosinophil granules isolated from guinea pig exudates.. Arch Biochem Biophys 1973 Jun;156(2):383-9.
Citations
This article has been cited 8 times.- van Dalen CJ, Winterbourn CC, Kettle AJ. Mechanism of nitrite oxidation by eosinophil peroxidase: implications for oxidant production and nitration by eosinophils.. Biochem J 2006 Mar 15;394(Pt 3):707-13.
- van Dalen CJ, Kettle AJ. Substrates and products of eosinophil peroxidase.. Biochem J 2001 Aug 15;358(Pt 1):233-9.
- Portmann P, Köppel P, Jörg A. An improved method for the isolation of eosinophilic leukocytes from normal human blood.. Experientia 1980 Jan 15;36(1):139-41.
- . Cells and inflammation: modern trends and technical outlook.. Klin Wochenschr 1984 May 15;62(10):479-503.
- Henderson WR, Chi EY, Jörg A, Klebanoff SJ. Horse eosinophil degranulation induced by the ionophore A23187. Ultrastructure and role of phospholipase A2.. Am J Pathol 1983 Jun;111(3):341-9.
- Jörg A, Henderson WR, Murphy RC, Klebanoff SJ. Leukotriene generation by eosinophils.. J Exp Med 1982 Feb 1;155(2):390-402.
- Lehnert BE, Steinkamp JA. Identification and isolation of subpopulations of pleural cells by multiparameter flow cytometry.. Cell Biophys 1986 Jun;8(3):201-12.
- Freiburghaus J, Jörg A. Isolation of bovine eosinophils and characterization of their leukotriene formation.. Agents Actions 1990 Aug;31(1-2):16-22.
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