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Virus research1994; 32(3); 391-399; doi: 10.1016/0168-1702(94)90087-6

A rapid method for the analysis of influenza virus genes: application to the reassortment of equine influenza virus genes.

Abstract: We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable for determination of partial nucleotide sequences which can be used to search nucleotide sequence databases and rapidly map the genetic origin of each segment. We illustrate the use of the method by analysing genetic reassortment in H7N7 equine influenza viruses.
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Publication Date: 1994-06-01 PubMed ID: 7521550DOI: 10.1016/0168-1702(94)90087-6Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article introduces a quick method for analyzing the genes of influenza virus by using reverse transcription and polymerase chain reaction. This method was applied to examine gene reassortment in H7N7 equine influenza viruses.

Method for Characterizing Influenza Virus Genes

  • The researchers describe a method for fast genetic characterization of influenza virus genes. This technique involves using reverse transcription (the process of creating a single stranded RNA from a DNA molecule) together with amplification by polymerase chain reaction (PCR, a method used to rapidly make millions to billions of copies of a specific DNA sample), a process known as RT/PCR.
  • This RT/PCR method was done on all virus segments at the same time, a technique known as multiplex RT/PCR.
  • The primers for the RT/PCR were based on the conserved terminal sequences that are common in various influenza strains.

Usage of the Developed Method

  • The RT/PCR products were found to be useful for determining partial nucleotide sequences. Nucleotides are organic molecules that are the basic structural units of nucleic acids such as DNA and RNA.
  • It allows researchers to efficiently pinpoint the genetic origin of each segment by searching nucleotide sequence databases.
  • The method enables scientists to quickly examine the genetic structure of viruses, opening up the potential for the development of treatments.

Application on H7N7 Equine Influenza Viruses

  • In this study, the described method is specifically utilized in the investigation of genetic reassortment in H7N7 equine influenza viruses.
  • Genetic reassortment in viruses refers to the process where viruses exchange or mix their genetic material.
  • Understanding how gene reassortment happens in the H7N7 equine influenza virus could potentially help in predicting and controlling future outbreaks of this virus in horses.

Cite This Article

APA
Adeyefa CA, Quayle K, McCauley JW. (1994). A rapid method for the analysis of influenza virus genes: application to the reassortment of equine influenza virus genes. Virus Res, 32(3), 391-399. https://doi.org/10.1016/0168-1702(94)90087-6

Publication

Virus research
ISSN: 0168-1702
NlmUniqueID: 8410979
Country: Netherlands
Language: English
Volume: 32
Issue: 3
Pages: 391-399

Researcher Affiliations

Adeyefa, C A
  • AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
Quayle, K
    McCauley, J W

      MeSH Terms

      • Base Sequence
      • Conserved Sequence
      • DNA Primers
      • Databases, Factual
      • Genes, Viral
      • Influenza A virus / genetics
      • Molecular Sequence Data
      • Orthomyxoviridae / genetics
      • Polymerase Chain Reaction / methods
      • RNA, Viral / analysis
      • RNA, Viral / genetics
      • RNA-Directed DNA Polymerase
      • Reassortant Viruses / genetics
      • Sequence Alignment
      • Sequence Analysis, DNA
      • Sequence Homology, Nucleic Acid

      Citations

      This article has been cited 11 times.
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      10. Adeyefa CA, McCauley JW, Tomori O. Mutational changes in the hemagglutinin of equine H3 influenza viruses result in the introduction of a glycosylation site which enhances the infectivity of the viruses. Folia Microbiol (Praha) 1997;42(4):390-4.
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        doi: 10.1017/s0950268800001552pubmed: 8870635google scholar: lookup
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