A selective increase in circulating inhibin and inhibin pro-alphaC at the time of ovulation in the mare.
Abstract: The relationship between a selective increase in circulating immunoreactive (ir)-inhibin and the time of ovulation was investigated in mares. Concentrations of plasma ir-inhibin were measured every 4 h during the periovulatory period. Inhibin pro-alphaC, a precursor protein of the inhibin alpha-subunit, was also measured. The changes in ir-inhibin and inhibin pro-alphaC in circulation were parallel. Concentrations of both ir-inhibin and inhibin pro-alphaC in the plasma increased at the same time when ovulatory follicles ruptured, and the peak levels of circulating ir-inhibin and inhibin pro-alphaC were maintained for 4-8 h. There was no selective increase in plasma concentrations of estradiol-17beta during the process of ovulation. These results suggest that the selective increase in ir-inhibin and inhibin pro-alphaC was caused by the absorption of follicular fluid after the rupture of ovulatory follicles. These results also suggest that the measuring of plasma concentrations of ir-inhibin or inhibin pro-alphaC in mares might be a useful method for detecting the time of ovulation.
Publication Date: 1999-11-24 PubMed ID: 10567014DOI: 10.1152/ajpendo.1999.277.5.E870Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research paper discusses the relationship between the increased levels of Immunoreactive Inhibin (ir-inhibin) and Inhibin Pro-alphaC (a precursor protein of inhibin alpha-subunit) circulating in the blood and the occurrence of ovulation in mares. The study infers that measuring these protein levels can potentially serve as an effective method to accurately detect ovulation time in mares.
Research Methodology
- The research involved the examination of the relationship between circulating Immunoreactive Inhibin (ir-inhibin) and Inhibin Pro-alphaC and the timing of ovulation in mares.
- Plasma concentrations of ir-inhibin were measured every 4 hours during the period surrounding ovulation, also known as the periovulatory period.
- Levels of Inhibin Pro-alphaC, a precursor protein for the inhibin alpha-subunit, were also measured during this time period.
Results and Findings
- The research found that levels of both ir-inhibin and Inhibin Pro-alphaC increase in correspondence with the rupture of ovulatory follicles. These peaks in protein levels were maintained for 4-8 hours.
- The study did not find a similar increase in plasma concentrations of estradiol-17beta during ovulation, suggesting a selective increase of ir-inhibin and Inhibin Pro-alphaC.
- The changes in concentrations of circulating ir-inhibin and inhibin pro-alphaC were found to be parallel, suggesting a related biological process.
- The study concluded that this increase in both ir-inhibin and inhibin pro-alphaC could be due to the absorption of follicular fluid that occurs after the rupture of ovulatory follicles.
Significance and Implications
- The research’s findings suggest that measuring plasma concentrations of ir-inhibin or inhibin pro-alphaC could be an effective method for detecting ovulation in mares. This would potentially allow for more precise breeding practices and enhanced management of reproduction within the species.
Cite This Article
APA
Nagaoka K, Nambo Y, Nagamine N, Nagata SI, Tanaka Y, Shinbo H, Tsunoda N, Taniyama H, Watanabe G, Groome NP, Taya K.
(1999).
A selective increase in circulating inhibin and inhibin pro-alphaC at the time of ovulation in the mare.
Am J Physiol, 277(5), E870-E875.
https://doi.org/10.1152/ajpendo.1999.277.5.E870 Publication
Researcher Affiliations
- Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
MeSH Terms
- Animals
- Biomarkers
- Breeding
- Enzyme-Linked Immunosorbent Assay / methods
- Female
- Follicle Stimulating Hormone / blood
- Horses / physiology
- Inhibins / analysis
- Inhibins / blood
- Luteinizing Hormone / blood
- Ovulation / physiology
- Progesterone / blood
- Protein Precursors / analysis
- Protein Precursors / blood
- Radioimmunoassay
Citations
This article has been cited 4 times.- Leemans B, Bromfield EG, Stout TAE, Vos M, Van Der Ham H, Van Beek R, Van Soom A, Gadella BM, Henning H. Developing a reproducible protocol for culturing functional confluent monolayers of differentiated equine oviduct epithelial cells†. Biol Reprod 2022 Apr 26;106(4):710-729.
- Toishi Y, Tsunoda N, Kume K, Nagaoka K, Watanabe G, Taya K. PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum. J Reprod Dev 2016 Dec 20;62(6):631-634.
- Medan MS, Nambo Y, Nagamine N, Shinbo H, Watanabe G, Groome N, Taya K. Plasma concentrations of ir-inhibin, inhibin A, inhibin pro-alphaC, FSH, and estradiol-17beta during estrous cycle in mares and their relationship with follicular growth. Endocrine 2004 Oct;25(1):7-14.
- Leemans B, Gadella BM, Marchand JHEAM, Van Soom A, Stout TAE. Induction of in vivo-like ciliation in confluent monolayers of re-differentiated equine oviduct epithelial cells†. Biol Reprod 2024 Sep 14;111(3):580-599.
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