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Journal of applied physiology (Bethesda, Md. : 1985)2017; 124(4); 860-865; doi: 10.1152/japplphysiol.00461.2017

A time-course evaluation of inflammatory and oxidative markers following high-intensity exercise in horses: a pilot study.

Abstract: Exercise is a physiological stress resulting in reactive oxygen species and inflammatory mediators, the accumulation of which are thought to contribute to degenerative articular diseases. The horse is of particular interest in this regard as equine athletes are frequently exposed to repetitive bouts of high-intensity exercise. The purpose of this study was to provide a detailed description of the response of articular and systemic oxidative and inflammatory biomarkers following high-intensity, exhaustive exercise in horses. A group of horses (Ex) underwent repeated bouts of high-intensity exercise, at a target heart rate of 180 beats/min, until voluntary exhaustion. Baseline plasma and synovial fluid (SF) samples were taken 24 h before exercise and then at 0.5, 1, 2, 4, 8, and 24 h following exercise cessation. This time course was repeated in a group of nonexercised control horses (Co). Plasma and SF samples were analyzed for prostaglandin E (PGE), nitric oxide (NO), total antioxidant status (TAS), and glycosaminoglycans (GAG). The Ex group had significantly higher plasma NO at 0.5, 1, and 2 h; and higher plasma PGE at 0.5 and 1 h compared with Co. SF PGE and GAG were also higher in Ex horses at 8 h compared with Co. It is concluded that high-intensity exercise in horses results in a rapid increase in systemic oxidative and inflammatory markers from 0.5 to 2 h after exercise, which is followed by local articular inflammation and cartilage turnover at 8 h postexercise. NEW & NOTEWORTHY In horses, the influence of exercise systemically and within the articular space remains unclear and requires further detailed characterization. In this study, we identify that an acute bout of high-intensity exercise in horses induces systemic inflammation and oxidative stress within 30 min of exercise cessation, which lasts for ~2 h. Articular inflammation and cartilage turnover were also be observed within the equine carpal joint 8 h following exercise completion.
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Publication Date: 2017-10-26 PubMed ID: 29074709PubMed Central: PMC5972457DOI: 10.1152/japplphysiol.00461.2017Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studies the effects of high-intensity exercise on horses, specifically focusing on oxidative and inflammatory markers in response to such physical stress. The study reveals that intense exercise leads to an immediate and rapid increase in these markers, which could possibly contribute to degenerative articular diseases over time.

Study Overview

  • The study aims to understand the effect of high-intensity exercise on horses. The experiment was directed towards unveiling the response of articular and systemic oxidative and inflammatory biomarkers to such exercise.
  • A group of horses was made to exercise intensively until they voluntarily exhausted themselves, the target heart rate being 180 beats/min. The researchers collected Plasma and Synovial fluid (SF) samples before the exercise, and at specific intervals after it.
  • A control group of non-exercising horses was also maintained for a comparative time-course study.

Methodology and Findings

  • The collected plasma and SF samples were analyzed for specific markers including Prostaglandin E (PGE), Nitric oxide (NO), Total antioxidant status (TAS), and Glycosaminoglycans (GAG).
  • The study found that the high-intensity exercise horses had significantly higher plasma NO at 0.5, 1, and 2 hours, and higher plasma PGE at 0.5 and 1 hour compared to the control group. Also, SF PGE and GAG were found to be higher in Ex horses after 8 hours.

Summary and Conclusion

  • The data suggests that high-intensity exercise in horses leads to a swift rise in systemic oxidative and inflammatory markers, which lasts for about 2 hours after exercise. This was followed by local articular inflammation and cartilage turnover about 8 hours postexercise.
  • The study adds to the existing body of knowledge about the systemic and articular effects of exercise in horses and provides the basis for further examination into how these reactions can contribute to degenerative articular diseases in equine athletes.

Cite This Article

APA
MacNicol JL, Lindinger MI, Pearson W. (2017). A time-course evaluation of inflammatory and oxidative markers following high-intensity exercise in horses: a pilot study. J Appl Physiol (1985), 124(4), 860-865. https://doi.org/10.1152/japplphysiol.00461.2017

Publication

Journal of applied physiology (Bethesda, Md. : 1985)
ISSN: 1522-1601
NlmUniqueID: 8502536
Country: United States
Language: English
Volume: 124
Issue: 4
Pages: 860-865

Researcher Affiliations

MacNicol, Jennifer L
  • Department of Animal Biosciences, University of Guleph , Guelph, Ontario , Canada.
Lindinger, Michael I
  • Nutraceutical Alliance, Campbellville, Ontario , Canada.
Pearson, Wendy
  • Department of Animal Biosciences, University of Guleph , Guelph, Ontario , Canada.

MeSH Terms

  • Animals
  • Antioxidants / metabolism
  • Dinoprostone / blood
  • Female
  • Glycosaminoglycans / blood
  • Horses / blood
  • Inflammation / etiology
  • Male
  • Nitric Oxide / blood
  • Oxidative Stress
  • Physical Conditioning, Animal / adverse effects
  • Pilot Projects
  • Synovial Fluid / metabolism

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Citations

This article has been cited 10 times.
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