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A tracheoscopic technique for obtaining uncontaminated lower airway secretions for bacterial culture in the horse.
Abstract: Transtracheal aspirate (TTA) aerobic microbiology culture results from 30 ponies were compared to protected catheter brush (PCB) culture results obtained from the same ponies. The PCB procedure resulted in significantly fewer (P less than 0.05) isolates than those obtained by the TTA procedure and the PCB procedure resulted in significantly more samples with no bacteria growth than the TTA procedure. To address the possibility that the volume of tracheobronchial secretion obtained by PCB was insufficient as a microbiological sample, a sterile, double-sheathed, protected aspiration catheter (PAC) was developed. Comparisons were made between TTA and PAC aerobic microbiology culture results from 18 pneumonic foals and weanlings. There were no significant differences between the PAC and TTA procedures with respect to the number of bacteria isolates. It was concluded that the PAC technique may be used as an alternative to TTA for isolation of equine pulmonary bacteria and may be the preferred procedure because of the reduced risk of complications associated with the TTA.
Publication Date: 1990-05-01 PubMed ID: 2361502DOI: 10.1111/j.2042-3306.1990.tb04239.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article is about a comparative study between two techniques, Transtracheal Aspirate (TTA) and Protected Catheter Brush (PCB), used for obtaining bacterial samples from horses’ airways. It was found that a new sterile technique using a Protected Aspiration Catheter (PAC) resulted in similar findings as the TTA method but with minimized risk.
Introduction
The study focuses on finding an alternative and less risky method to the Transtracheal Aspirate (TTA) technique used for obtaining bacterial culture samples from the lower airways of ponies. The alternative method being used for comparisons is the Protective Catheter Brush (PCB) method.
Methods
- The bacterial culture results from the TTA method acquired from 30 ponies were compared to the results obtained by using the PCB method on the same ponies.
- The comparison was mainly based on the number of bacterial isolates obtained and the number of samples showing no bacterial growth.
- To address the concern regarding the volume of the tracheobronchial secretion obtained by the PCB method, a new technique was introduced: a Protected Aspiration Catheter (PAC).
Findings
- It was found that the PCB method resulted in significantly fewer isolates than the TTA method.
- The PCB method yielded more samples with no bacterial growth compared to the TTA method.
- Comparisons were then made between TTA and PAC results obtained from 18 foals and weanlings suffering from pneumonia.
- It was observed that the number of bacterial isolates obtained from both methods did not have any significant differences.
Conclusion
- From the study, it was concluded that the PAC technique can serve as an efficient alternative to the TTA method for obtaining equine pulmonary bacteria samples.
- It was also suggested that the PAC method might be the preferred procedure because it reduces the risk of complications associated with the TTA method, making it a safer choice.
Cite This Article
APA
Darien BJ, Brown CM, Walker RD, Williams MA, Derksen FJ.
(1990).
A tracheoscopic technique for obtaining uncontaminated lower airway secretions for bacterial culture in the horse.
Equine Vet J, 22(3), 170-173.
https://doi.org/10.1111/j.2042-3306.1990.tb04239.x Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314.
MeSH Terms
- Animals
- Bacteria / growth & development
- Bacteria / isolation & purification
- Bacterial Infections / microbiology
- Bacterial Infections / veterinary
- Bronchi / microbiology
- Bronchoalveolar Lavage Fluid / microbiology
- Bronchoscopy / veterinary
- Female
- Horse Diseases / microbiology
- Horses
- Male
- Pneumonia / microbiology
- Pneumonia / veterinary
- Trachea / microbiology
Citations
This article has been cited 4 times.- Shawaf T, Almubarak A, Alhumam N, Almathen F, Hussen J. Cytological analysis of tracheal wash and bronchoalveolar lavage fluid in health and respiratory disease in dromedary camels. PeerJ 2021;9:e11723.
- Naylor JM, Clark EG, Clayton HM. Chronic obstructive pulmonary disease: Usefulness of clinical signs, bronchoalveolar lavage, and lung biopsy as diagnostic and prognostic aids. Can Vet J 1992 Sep;33(9):591-8.
- Giguère S, Hernandez J, Gaskin J, Prescott JF, Takai S, Miller C. Performance of five serological assays for diagnosis of Rhodococcus equi pneumonia in foals. Clin Diagn Lab Immunol 2003 Mar;10(2):241-5.
- Labonville M, Higgins R, Lavoie JP. [Comparison of direct smear observations and bacteriologic culture results carried out on equine tracheal secretions]. Can Vet J 2001 Aug;42(8):623-6.
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