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Home / Equine Research Bank / Arch Virol / A type-specific conformational epitope on the nucleocapsid o...
Archives of virology1993; 132(1-2); 133-144; doi: 10.1007/BF01309848

A type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis.

Abstract: A type-specific monoclonal antibody was produced by immunizing mice with purified equid herpesvirus-1 (EHV-1). The EHV-1 specific mAb reacted with all the EHV-1 strains tested so far by indirect ELISA, immunofluorescence, and immunoperoxidase tests. No reactions were detected with the EHV-4, EHV-2, or EHV-3 strains tested. The indirect immunofluorescence and immunoperoxidase tests showed that the nuclei of infected cells were predominantly stained by this mAb. Triton treatment of the virus and immunogold labeling experiments indicated that the nucleocapsid of EHV-1 was the target antigen of the mAb. Preliminary results indicated that this mAb might be a useful tool in detecting specific antibody in horses that have been exposed to EHV-1. In a blocking ELISA, antibodies in sera from hamsters, mice and a foal which had been exposed to EHV-1 were differentiated from those in sera of animals exposed to EHV-4.
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Publication Date: 1993-01-01 PubMed ID: 7688948DOI: 10.1007/BF01309848Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study produced a unique monoclonal antibody against equid herpesvirus-1 (EHV-1) by immunizing mice and demonstrated its potential use in diagnosing EHV-1 infections in horses. This antibody specifically targets the nucleocapsid of EHV-1 and can differentiate EHV-1 from other strains of equine herpesvirus.

Process of Monoclonal Antibody Production

  • The research started by immunizing mice with purified EHV-1, which led to the production of a specific monoclonal antibody (mAb). This mAb production is an immune response of the mice to the EHV-1 virus.
  • Monoclonal antibodies are identical immune cells that are cloned from a single parent cell. They react to a specific antigen, in this case, a protein present on the EHV-1 virus.

Detection and Reaction of Monoclonal Antibody

  • The EHV-1 specific mAb recognized and reacted with every EHV-1 strain tested by indirect ELISA, immunofluorescence, and immunoperoxidase tests, demonstrating its specificity to EHV-1.
  • No reactions were observed with other equid herpesviruses such as EHV-4, EHV-2, or EHV-3, underlining the mAb’s specificity.
  • The indirect immunofluorescence and immunoperoxidase tests exhibited that the mAb predominantly stained the nuclei of cells infected with EHV-1.

Target of the Monoclonal Antibody

  • After treating the EHV-1 virus with Triton (a detergent used to disrupt cell membranes), the researchers identified that the nucleocapsid (the protein casing of the viral genome) of the virus was the target of the mAb.
  • Immunogold labeling experiments, which utilizes gold particles to visualize sites of antibody-antigen interaction, further confirmed this.

Potential Diagnostic Use

  • The monoclonal antibody could potentially serve as a tool in detecting specific antibodies in horses exposed to EHV-1.
  • Initial results suggested it could differentiate between EHV-1 and EHV-4 in sera from various animals (hamsters, mice, and a foal), through a blocking ELISA (a test that detects the presence of specific antibodies).

Cite This Article

APA
van de Moer A, Rice M, Wilks CR. (1993). A type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis. Arch Virol, 132(1-2), 133-144. https://doi.org/10.1007/BF01309848

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 132
Issue: 1-2
Pages: 133-144

Researcher Affiliations

van de Moer, A
  • Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
Rice, M
    Wilks, C R

      MeSH Terms

      • Animals
      • Antibodies, Monoclonal / biosynthesis
      • Antibodies, Monoclonal / immunology
      • Antibodies, Viral / biosynthesis
      • Antibodies, Viral / immunology
      • Capsid / immunology
      • Cells, Cultured
      • Cricetinae
      • Cross Reactions
      • Epitopes
      • Female
      • Herpesviridae Infections / diagnosis
      • Herpesviridae Infections / veterinary
      • Herpesvirus 1, Equid / immunology
      • Horse Diseases / diagnosis
      • Horse Diseases / microbiology
      • Horses
      • Immunologic Techniques
      • Mice
      • Mice, Inbred BALB C
      • Rabbits
      • Serologic Tests

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      Citations

      This article has been cited 1 times.
      1. Singh BK, Ahuja S, Gulati BR. Development of a neutralizing monoclonal antibody-based blocking ELISA for detection of equine herpesvirus 1 antibodies.. Vet Res Commun 2004 Jul;28(5):437-46.
        doi: 10.1023/b:verc.0000034996.18533.90pubmed: 15379438google scholar: lookup
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