Acid phosphatase activity in mouse brain infected with Venezuelan equine encephalomyelitis virus.
Abstract: The mode of development of Venezuelan equine encephalomyelitis virus and the activity of acid phosphatase in the central nervous system of newborn mice were investigated. Precursor particles appeared to be formed in masses of viroplasm, migrating to the membrane of the Golgi cisterns and vacuoles or to the plasma membrane and being transformed into mature viral particles by budding. Mature viral particles were also found in the lumen of the blood vessels and around the myelin sheath of axons. Increased number of Golgi complexes and depletion of polysomes were the main ultrastructural alterations of the nerve cells. Acid phosphatase activity was found to be increased in the Golgi cisterns, vacuoles, and lysosomes of nerve cells. The presence of acid phosphatase activity in the rough endoplasmic reticulum and perinuclear cisterns suggests increased production of the enzyme in the nerve cells infected with Venezuelan equine encephalomyelitis virus.
Publication Date: 1971-08-01 PubMed ID: 4107246PubMed Central: PMC356235DOI: 10.1128/JVI.8.2.232-241.1971Google Scholar: Lookup
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- Journal Article
Summary
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The study investigated how the Venezuelan equine encephalomyelitis virus develops and increases the activity of acid phosphatase in the central nervous system of newborn mice.
Key Findings
- The research revealed that the Venezuelan equine encephalomyelitis virus seems to facilitate its own development by forming precursor particles in masses of a substance called viroplasm. These particles then journey to different parts of the cell—like the membrane of Golgi cisterns and vacuoles, or the plasma membrane—where they transform into mature viral particles through a process known as budding.
- Mature viral particles were also located within the lumen of the blood vessels and around the myelin sheath of axons, indicating how the virus might travel and spread within the host.
- On a cellular level, the nerve cells exhibited main ultrastructural changes characterized by abundant formation of Golgi complexes (structures involved in protein processing) and a depletion of polysomes (clusters of ribosomes that synthesize multiple proteins).
Acid Phosphatase Activity
- The study also investigated acid phosphatase activity, an enzyme which can break down proteins, in the central nervous system of newborn mice infected with the Venezuelan equine encephalomyelitis virus.
- It was found that this activity was increased in the Golgi cisterns, vacuoles, and lysosomes of nerve cells, suggesting that the virus impacts the normal functioning of these cells.
- This increased acid phosphatase activity could be a result of the virus inducing enhanced production of the enzyme in the nerve cells. This is hinted at by the detection of acid phosphatase activity not just in the usual compartments where it is typically found, but also in abnormalities like the rough endoplasmic reticulum and perinuclear cisterns.
Implications of the Study
- The findings of this study are significant because they offer insights into how the Venezuelan equine encephalomyelitis virus develops and propagates within the host, contributing to the understanding of this particular viral infection.
- Notably, the changes in acid phosphatase activity could be a potential biomarker for monitoring the infection as well as a potential target for therapeutic intervention.
Cite This Article
APA
Garcia-Tamayo J.
(1971).
Acid phosphatase activity in mouse brain infected with Venezuelan equine encephalomyelitis virus.
J Virol, 8(2), 232-241.
https://doi.org/10.1128/JVI.8.2.232-241.1971 Publication
Researcher Affiliations
MeSH Terms
- Acid Phosphatase / metabolism
- Animals
- Animals, Newborn
- Blood Vessels
- Brain / blood supply
- Brain / enzymology
- Brain / microbiology
- Brain / pathology
- Encephalitis Virus, Venezuelan Equine / growth & development
- Encephalitis Virus, Venezuelan Equine / pathogenicity
- Encephalitis Viruses / pathogenicity
- Encephalomyelitis, Equine / enzymology
- Encephalomyelitis, Equine / microbiology
- Encephalomyelitis, Equine / pathology
- Golgi Apparatus
- Histocytochemistry
- Mice
- Mice, Inbred Strains
- Microscopy, Electron
- Neuroglia
- Neurons
- Staining and Labeling
- Virus Replication
References
This article includes 14 references
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Citations
This article has been cited 2 times.- García-Tamayo J, Esparza J, Martínez AJ. Placental and fetal alterations due to Venezuelan equine encephalitis virus in rats.. Infect Immun 1981 May;32(2):813-21.
- Veckenstedt A, Wagner M. Virological and immunofluorescent studies on the multiplication of Sindbis virus in L cells.. Arch Gesamte Virusforsch 1973;42(2):144-53.
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