Acinetobacter gandensis sp. nov. isolated from horse and cattle.
Abstract: We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, % G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4 %), Acinetobacter haemolyticus (97.7 %), and Acinetobacter schindleri (97.2 %). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6 %), A. bouvetii NIPH 2281 (88.6 %) and A. schindleri CIP 107287T (87.3 %). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C12 : 0 3-OH, C12 : 0, C16 : 0, C18 : 1ω9c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84 % with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 % and 20 %, respectively. The DNA G+C content of strain UG 60467(T) was 39.6 mol%. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) ( = ANC 4275(T) = LMG 27960(T) = DSM 28097(T)).
© 2014 IUMS.
Publication Date: 2014-09-15 PubMed ID: 25225259DOI: 10.1099/ijs.0.068791-0Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research documented the discovery and characterization of a new species of the Acinetobacter genus, referred to as Acinetobacter gandensis sp. nov., that was sourced from horse and cattle waste.
Background of the Research
- The isolation and study of a previously unidentified strain of a bacterium belonging to the Acinetobacter genus initiated the research. This genus is of particular interest due to its role in developing antimicrobial resistance.
- The researchers discovered this new bacterial strain in the waste materials of horses in a Veterinary Medicine school in Ghent University, Belgium. This bacterium has the OXA-23 carbapenemase, an enzyme that allows bacteria to resist the effects of certain antibiotics.
Methodology and Analysis
- The researchers performed an extensive characterisation of this new strain, incorporating six other strains for reliable comparability.
- They made use of tested methods such as DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, percent G+C determination, MALDI-TOF MS, and fatty acid profile analysis.
- The gene sequence and protein level analyses (16S rRNA, rpoB, and MALDI-TOF MS) of the new strain revealed a high similarity with three other Acinetobacter species: A. bouvetii, A. haemolyticus, and A. schindleri.
- The fatty acid composition, important for identifying bacteria, also supported that this new strain belonged to the Acinetobacter genus.
Conclusion and Results
- Data derived from the comparative analysis established the unique identity of the new Acinetobacter strain.
- A considerable DNA-DNA relatedness (84%) between strains UG 60467(T) and UG 60716 confused their respective identities, but their low relatedness with A. schindleri LMG 19576(T) of 17% and 20%, confirmed their novelty.
- They declared the new species’ name to be Acinetobacter gandensis sp. nov. and designated UG 60467(T) as the type strain.
Cite This Article
APA
Smet A, Cools P, Krizova L, Maixnerova M, Sedo O, Haesebrouck F, Kempf M, Nemec A, Vaneechoutte M.
(2014).
Acinetobacter gandensis sp. nov. isolated from horse and cattle.
Int J Syst Evol Microbiol, 64(Pt 12), 4007-4015.
https://doi.org/10.1099/ijs.0.068791-0 Publication
Researcher Affiliations
- Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan133, 9820 Merelbeke, Belgium.
- Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium.
- Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
- Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
- Research Group Proteomics, Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
- Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan133, 9820 Merelbeke, Belgium.
- Laboratoire de Bactériologie, Institut de Biologie en Santé - PBU, CHU, 4 rue Larrey, 49933 Angers cedex, France.
- Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
- Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium.
MeSH Terms
- Acinetobacter / classification
- Acinetobacter / genetics
- Acinetobacter / isolation & purification
- Animals
- Bacterial Typing Techniques
- Base Composition
- Belgium
- Cattle / microbiology
- DNA, Bacterial / genetics
- Fatty Acids / chemistry
- Feces / microbiology
- Genes, Bacterial
- Horses / microbiology
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Phylogeny
- RNA, Ribosomal, 16S / genetics
- Sequence Analysis, DNA
Citations
This article has been cited 9 times.- Zhao Y, Zhu Y, Liu B, Mi J, Li N, Zhao W, Wu R, Holyoak GR, Li J, Liu D, Zeng S, Wang Y. Antimicrobial Susceptibility of Bacterial Isolates from Donkey Uterine Infections, 2018-2021. Vet Sci 2022 Feb 5;9(2).
- Baleeiro FCF, Ardila MS, Kleinsteuber S, Sträuber H. Effect of Oxygen Contamination on Propionate and Caproate Formation in Anaerobic Fermentation. Front Bioeng Biotechnol 2021;9:725443.
- Welter DK, Ruaud A, Henseler ZM, De Jong HN, van Coeverden de Groot P, Michaux J, Gormezano L, Waters JL, Youngblut ND, Ley RE. Free-Living, Psychrotrophic Bacteria of the Genus Psychrobacter Are Descendants of Pathobionts. mSystems 2021 Apr 13;6(2).
- Honeyman AS, Day ML, Spear JR. Regional fresh snowfall microbiology and chemistry are driven by geography in storm-tracked events, Colorado, USA. PeerJ 2018;6:e5961.
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- Krizova L, McGinnis J, Maixnerova M, Nemec M, Poirel L, Mingle L, Sedo O, Wolfgang W, Nemec A. Acinetobacter variabilis sp. nov. (formerly DNA group 15 sensu Tjernberg & Ursing), isolated from humans and animals. Int J Syst Evol Microbiol 2015 Mar;65(Pt 3):857-863.
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