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Home / Equine Research Bank / J Vet Med Sci / Actinomyces denticolens as a causative agent of actinomycosi...
The Journal of veterinary medical science2018; 80(11); 1650-1656; doi: 10.1292/jvms.18-0207

Actinomyces denticolens as a causative agent of actinomycosis in animals.

Abstract: The name "Actinomyces suis" was applied to each actinomycete isolate from swine actinomycosis by Grässer in 1962 and Franke in 1973. Nevertheless, this specific species was not included in the "Approved List of Bacterial Name" due to absence of the type cultures. Therefore, "Actinomyces suis" based on the description of Franke 1973 has been considered as "species incertae sedis". We isolated a number of Actinomyces strains from swine. The representative strains of them was designated as Chiba 101 that was closely similar to the description in "Actinomyces suis" reported by Franke in 1973. Interestingly, it was found that the biological characteristics of these strains were also very similar to those of Actinomyces denticolens. Furthermore, the average nucleotide identity (ANI) value between strain Chiba 101 and the type-strain of Actinomyces denticolens (=DSM 20671) was found to be 99.95%. Sequences of the housekeeping genes and 16S rRNA gene showed 100% homology. These results strongly suggested that "Actinomyces suis" Franke 1973 is the same species as Actinomyces denticolens. Since actinomycosis caused by Actinomyces denticolens have been demonstrated in horses recently, it is necessary to recognize that Actinomyces denticolens is the pathogenic actinomycetes in broader range of animals.
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Publication Date: 2018-09-14 PubMed ID: 30224576PubMed Central: PMC6261818DOI: 10.1292/jvms.18-0207Google Scholar: Lookup
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Summary

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The research explores the identity of a strain of actinomycete bacteria – previously labeled ‘Actinomyces suis’ – isolated from pigs and determines it to be a strain of Actinomyces denticolens. This information broadens our understanding of the range of animals in which Actinomyces denticolens can cause actinomycosis, a bacterial infection.

Actinomyces Consistency

  • This study started with the historical issue that “Actinomyces suis”, a name given by Grässer and Franke in the mid-20th century to a group of bacteria isolated from pigs, was left off the “Approved List of Bacterial Name”. This was due to the absence of ‘type cultures’, which are reference strains of organisms used in microbial systematics to define a species. This absence left “Actinomyces suis” as “species incertae sedis” – an uncertain species.
  • A number of Actinomyces strains were isolated from swine for this study, with one designated as Chiba 101 for its similarity to the description of “Actinomyces suis” given by Franke in 1973.

Biological Characteristics and Nucleotide Analysis

  • Upon further examination, the researchers found that the biological characteristics of these strains were very similar to those of Actinomyces denticolens, another species of Actinomyces.
  • A significant similarity was also discovered at the molecular level. The average nucleotide identity (ANI) between the Chiba 101 strain and the type-strain of Actinomyces denticolens was found to be nearly identical at 99.95%.
  • Additionally, when the team sequenced the bacteria’s ‘housekeeping’ genes and 16S rRNA gene – the reliable markers for bacterial phylogenetic studies – they found a 100% match between the two.

Implications

  • Such overwhelming similarity strongly suggested to the researchers that the so-called “Actinomyces suis” is actually Actinomyces denticolens.
  • The finding is important because Actinomyces denticolens has recently been found to cause actinomycosis in horses. With this new knowledge that A. denticolens, not some unique ‘A. suis,’ is also found in swine, the medical and veterinary fields must anticipate that Actinomyces denticolens can cause actinomycosis in a broader range of animals than previously thought.

Cite This Article

APA
Murakami S, Kobayashi T, Sekigawa Y, Torii Y, Kanesaki Y, Ishige T, Yokoyama E, Ishiwata H, Hamada M, Tamura T. (2018). Actinomyces denticolens as a causative agent of actinomycosis in animals. J Vet Med Sci, 80(11), 1650-1656. https://doi.org/10.1292/jvms.18-0207

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 80
Issue: 11
Pages: 1650-1656

Researcher Affiliations

Murakami, Satoshi
  • Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa 243-0034, Japan.
Kobayashi, Tomoko
  • Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa 243-0034, Japan.
Sekigawa, Yuriko
  • Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa 243-0034, Japan.
Torii, Yasushi
  • Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa 243-0034, Japan.
Kanesaki, Yu
  • Department of Genome Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Ishige, Taichiro
  • Department of Genome Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Yokoyama, Eiji
  • Chiba Prefectural Institute of Public Health, 666-2 Nitona, Chuo, Chiba, Chiba 260-8715, Japan.
Ishiwata, Hiroyuki
  • Technical Research Institute, Nishimatsu Construction Co., Ltd., 6-17-21 Shinbashi, Minato, Tokyo 105-0004, Japan.
Hamada, Moriyuki
  • Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Tamura, Tomohiko
  • Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.

MeSH Terms

  • Actinomyces / classification
  • Actinomyces / isolation & purification
  • Actinomycosis / microbiology
  • Actinomycosis / pathology
  • Actinomycosis / veterinary
  • Animals
  • Horse Diseases / microbiology
  • Horses
  • Molecular Typing
  • Palatine Tonsil / microbiology
  • RNA, Fungal
  • RNA, Ribosomal, 16S
  • Swine
  • Swine Diseases / microbiology
  • Swine Diseases / pathology

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Citations

This article has been cited 2 times.
  1. van den Wollenberg L, van Maanen C, Buter R, Janszen P, Rey F, van Engelen E. Detection and molecular characterization of Actinomyces denticolens causing lymph node abscessation in horses.. Front Vet Sci 2023;10:1225528.
    doi: 10.3389/fvets.2023.1225528pubmed: 37546341google scholar: lookup
  2. Hu D, Chao Y, Zhang B, Wang C, Qi Y, Ente M, Zhang D, Li K, Mok KM. Effects of Gasterophilus pecorum infestation on the intestinal microbiota of the rewilded Przewalski's horses in China.. PLoS One 2021;16(5):e0251512.
    doi: 10.1371/journal.pone.0251512pubmed: 33974667google scholar: lookup
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