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Veterinary record open2016; 3(1); e000161; doi: 10.1136/vetreco-2015-000161

Actinomyces denticolens colonisation identified in equine tonsillar crypts.

Abstract: Recently, submandibular abscesses associated with Actinomyces denticolens have been reported in horses. The actinomycotic clumps have been observed in the tonsillar crypts. The aim of this study was to demonstrate colonisation of A denticolens in equine tonsils. Twelve equine tonsils obtained from a slaughterhouse were divided into two parts for histopathological examination and for isolation of A denticolens. When actinomycotic clumps were found in these tonsillar crypts, immunohistochemistry using hyperimmune serum against A denticolens (DMS 20671) was performed on the serial sections. To determine whether Actinomyces-like bacteria isolated using immunoantigenic separation technique were A denticolens, the isolates were analysed for the 16S rRNA gene sequence. Actinomycotic clumps were found in the tonsillar crypts of 11 (91.7 per cent) horses. The clumps were of the saprophytic type accompanied with the feedstuffs, but a few clumps were surrounded by inflammatory cells. A denticolens antigens were immunodetected not only in the clumps of 11 (100 per cent) tonsils, but also in the tonsillar parenchyma. Six isolates obtained from four tonsils showed 99.7-99.9 per cent similarity to A denticolens in the 16S rRNA gene sequence. In horses, the colonisation sites of A denticolens are the tonsils, thus the authors suggest that the tonsils provide the intrinsic infection site for A denticolens.
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Publication Date: 2016-09-08 PubMed ID: 27651913PubMed Central: PMC5020674DOI: 10.1136/vetreco-2015-000161Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper reports on a study that found the bacteria Actinomyces denticolens in the tonsils of horses, suggesting that these locations may serve as a primary source for intrinsic infection with this bacteria in equine populations.

Research Overview

The researchers investigated the prevalence and potential infection sites for Actinomyces denticolens, a bacterium that has been observed in horses with submandibular abscesses. Their objective was to prove that A. denticolens colonizes the tonsils of horses.

  • They used twelve horse tonsils obtained from a slaughterhouse for their study.
  • These tonsils were divided for histopathological examination and for bacterial isolation.

Methodology & Results

When actinomycotic (relating to the genus of bacteria Actinomyces) clumps were found in the tonsillar crypts (the extensions or pockets in the tonsils), a specific type of immunohistochemistry was applied.

  • This technique used a hyperimmune serum specific against A. denticolens.
  • In 11 out of 12 horses (91.7%), actinomycotic clumps were found.
  • Immunodetection revealed A. denticolens antigens, not only within these clumps but also in the tonsillar parenchyma, the functional tissue of the tonsil.

After conducting an immunoantigenic separation technique, bacteria similar to Actinomyces were isolated from the tonsils.

  • Through molecular analysis (16S rRNA gene sequence), a high similarity (around 99.7-99.9%) was noted to A. denticolens across six isolated strains from four tonsils.

Conclusions

With these results, the authors argued that A. denticolens colonizes the tonsils in horses. They suggested the tonsils act as an intrinsic infection site for this specific bacterium. This revelation opens the potential for further research into effective treatments and prevention strategies for diseases and conditions associated with A. denticolens in horses.

Cite This Article

APA
Murakami S, Otaki M, Hayashi Y, Higuchi K, Kobayashi T, Torii Y, Yokoyama E, Azuma R. (2016). Actinomyces denticolens colonisation identified in equine tonsillar crypts. Vet Rec Open, 3(1), e000161. https://doi.org/10.1136/vetreco-2015-000161

Publication

Veterinary record open
ISSN: 2052-6113
NlmUniqueID: 101653671
Country: United States
Language: English
Volume: 3
Issue: 1
Pages: e000161
PII: e000161

Researcher Affiliations

Murakami, S
  • Department of Animal Science , Tokyo University of Agriculture , 1737 Funako, Atsugi Kanagawa 243-0034 , Japan.
Otaki, M
  • Department of Animal Science , Tokyo University of Agriculture , 1737 Funako, Atsugi Kanagawa 243-0034 , Japan.
Hayashi, Y
  • Department of Animal Science , Tokyo University of Agriculture , 1737 Funako, Atsugi Kanagawa 243-0034 , Japan.
Higuchi, K
  • Department of Animal Science , Tokyo University of Agriculture , 1737 Funako, Atsugi Kanagawa 243-0034 , Japan.
Kobayashi, T
  • Department of Animal Science , Tokyo University of Agriculture , 1737 Funako, Atsugi Kanagawa 243-0034 , Japan.
Torii, Y
  • Department of Animal Science , Tokyo University of Agriculture , 1737 Funako, Atsugi Kanagawa 243-0034 , Japan.
Yokoyama, E
  • Chiba Prefectural Institute of Public Health , 666-2 Nitona, Chuo, Chiba 260-8715 , Japan.
Azuma, R
  • Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, Atsugi Kanagawa 243-0034, Japan; 2-7-33 Higashi-tokura, Kokubunji-city, Tokyo 185-0002, Japan.

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Citations

This article has been cited 2 times.
  1. van den Wollenberg L, van Maanen C, Buter R, Janszen P, Rey F, van Engelen E. Detection and molecular characterization of Actinomyces denticolens causing lymph node abscessation in horses. Front Vet Sci 2023;10:1225528.
    doi: 10.3389/fvets.2023.1225528pubmed: 37546341google scholar: lookup
  2. Murakami S, Kobayashi T, Sekigawa Y, Torii Y, Kanesaki Y, Ishige T, Yokoyama E, Ishiwata H, Hamada M, Tamura T. Actinomyces denticolens as a causative agent of actinomycosis in animals. J Vet Med Sci 2018 Nov 9;80(11):1650-1656.
    doi: 10.1292/jvms.18-0207pubmed: 30224576google scholar: lookup
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