Active expression of matrix metalloproteinase-13 mRNA in the granulation tissue of equine superficial digital flexor tendinitis.
Abstract: The DNA microarray analysis for matrix metalloproteinase (MMP)-related mRNA expression in equine superficial digital flexor tendinitis indicated that mRNA level of MMP-13 was apparently up-regulated in the tendinitis as compared to normal tendon. In situ hybridization also revealed that fibroblastic cells proliferated in the granulation tissue generated in the tendinitis actively expressed MMP-13 mRNA. On the other hand, in normal tendon, a few fibroblastic cells and vascular components lied in the endotenon barely expressed its mRNA, but other cellular components in the tendon bundle were not positively hybridized. As mentioned above, MMP-13 but not other collagenases or gelatinases, may play an important role in tendon injuries in the racehorses.
Publication Date: 2007-07-06 PubMed ID: 17611361DOI: 10.1292/jvms.69.637Google Scholar: Lookup
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Summary
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The researchers found increased expression of matrix metalloproteinase-13 (MMP-13) mRNA in the healing tissue of horses suffering from superficial digital flexor tendinitis, compared to normal tendon tissue. This indicates a significant role of MMP-13 in tendon injuries in racehorses.
Exploring MMP-13 mRNA Expression
- The researchers used a DNA microarray analysis to examine the expression of MMP-related mRNA, specifically MMP-13, in the tissue of horses with superficial digital flexor tendinitis.
- They found that the mRNA level of MMP-13 was increased in the tendon injury as compared to the normal tendon tissue.
In Situ Hybridization
- The team also used a technique called in situ hybridization to locate the expression of MMP-13 mRNA within the tissues.
- They discovered that fibroblastic cells, which proliferate in the healing tissue (granulation tissue) generated in the tendinitis, actively expressed MMP-13 mRNA.
Normal Tendon vs Tendon with Tendinitis
- In normal tendon tissue, MMP-13 mRNA expression was at a minimal level and was observed in only a few fibroblastic cells and vascular components in the endotenon.
- Meanwhile, in tendinitis stricken tendon tissue, there was a significant increase in MMP-13 mRNA expression, particularly in fibroblastic cells proliferating in the healing (granulation) tissue.
Role of MMP-13 in Tendon Injuries
- The significantly upregulated expression of MMP-13 mRNA observed in the tissue affected by tendinitis suggests that MMP-13 may play a crucial role in tendon injuries in racehorses.
- The increased presence of MMP-13 mRNA could indicate that it’s actively involved in the horse’s tendon injury repair and healing process.
- This study, therefore, shows potential for future investigations into therapeutic applications targeting MMP-13 for improving tendon injury treatment in horses.
Cite This Article
APA
Nomura M, Hosaka Y, Kasashima Y, Ueda H, Takehana K, Kuwano A, Arai K.
(2007).
Active expression of matrix metalloproteinase-13 mRNA in the granulation tissue of equine superficial digital flexor tendinitis.
J Vet Med Sci, 69(6), 637-639.
https://doi.org/10.1292/jvms.69.637 Publication
Researcher Affiliations
- Laboratory of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
MeSH Terms
- Animals
- Gene Expression Regulation
- Granulation Tissue / metabolism
- Horse Diseases / metabolism
- Horses
- Male
- Matrix Metalloproteinase 13 / metabolism
- RNA, Messenger / metabolism
- Tendinopathy / metabolism
- Tendons / enzymology
Citations
This article has been cited 7 times.- James S, Daffy J, Cook J, Samiric T. Short-Term Exposure to Ciprofloxacin Reduces Proteoglycan Loss in Tendon Explants. Genes (Basel) 2022 Nov 25;13(12).
- Doll CU, Niebert S, Burk J. Mesenchymal Stromal Cells Adapt to Chronic Tendon Disease Environment with an Initial Reduction in Matrix Remodeling. Int J Mol Sci 2021 Nov 26;22(23).
- Biasutti S, Dart A, Smith M, Blaker C, Clarke E, Jeffcott L, Little C. Spatiotemporal variations in gene expression, histology and biomechanics in an ovine model of tendinopathy. PLoS One 2017;12(10):e0185282.
- Spiesz EM, Thorpe CT, Chaudhry S, Riley GP, Birch HL, Clegg PD, Screen HR. Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise. J Orthop Res 2015 Jun;33(6):889-97.
- Juneja SC, Schwarz EM, O'Keefe RJ, Awad HA. Cellular and molecular factors in flexor tendon repair and adhesions: a histological and gene expression analysis. Connect Tissue Res 2013;54(3):218-26.
- Omae H, Zhao C, Sun YL, Zobitz ME, Moran SL, Amadio PC. The effect of tissue culture on suture holding strength and degradation in canine tendon. J Hand Surg Eur Vol 2009 Oct;34(5):643-50.
- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
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