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Active hyaluronidase 2 expression in the granulation tissue formed in the healing process of equine superficial digital flexor tendonitis.
Abstract: Upregulation of hyaluronidase 2 (HYAL2), one of somatic hyaluronidase (HAase), was demonstrated in granulation tissue during the healing of equine superficial digital flexor tendon injuries. The activity of HAase was assessed by hyaluronan (HA)-containing gel zymography and in situ zymography using frozen sections obtained from normal and injured tendon tissues. Elevated HAase activity was identified in the extract from the tendinopathic tissues, with lower levels of the activity in normal tendons. In situ zymography using fluorescently-labeled HA demonstrated HAase activity in the granulation tissue formed in the injured region. In addition, in situ hybridization analysis indicated that fibroblastic cells in the granulation tissue of the injured tendon actively expressed HYAL2 but not HYAL1. Quantitative RT-PCR further confirmed a higher level of amplicons corresponding to HYAL2 in tendonitis-derived samples. These results suggest that elevated HYAL2 activity in the granulation tissue could participate in controlling the healing process in equine tendonitis.
Publication Date: 2012-10-05 PubMed ID: 23047331DOI: 10.1292/jvms.12-0318Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study looks into the elevated expression of hyaluronidase 2 (HYAL2) in the healing process of horse tendon injuries. Elevated levels of this enzyme are found in the granulation tissue, a form of new tissue that forms on the surfaces of wounds during the healing period, suggesting a potential role in controlling the healing process.
Research Background
- The research focuses on HYAL2, a type of somatic hyaluronidase (HAase). HYAL2 helps degrade hyaluronic acid, a substance important in tissue repair and the inflammatory response.
- HYAL2 becomes upregulated – increased in response to a trigger – in the granulation tissue during the healing process of equine tendon injuries. On the other hand, hyaluronidase 1 (HYAL1), another type of the enzyme, did not show such upregulation.
Methodology and Findings
- The research studied samples from both normal and injured tendons. HYAL2’s activity was analyzed by techniques called gel zymography and in situ zymography, which help detect enzyme activity.
- The study found that injured tendons showed increased HAase activity as compared to normal tendons.
- This heightened activity was particularly seen in the granulation tissue, suggesting HYAL2’s involvement in the healing process of tendon injuries.
- Findings from in situ hybridization revealed that fibroblastic cells, which are important in wound repair, also showed active HYAL2 expression in the granulation tissue of the injured tendon.
- Quantitative RT-PCR, a technique used to measure the amount of specific RNA, further confirmed that HYAL2 levels were higher in tendonitis-derived samples.
Implications
- The study suggests that an elevated level of HYAL2 in the granulation tissue might have a role in controlling the healing process in equine tendonitis.
- This opens up possibilities for further research into how HYAL2 may be utilized or targeted for effective treatment strategies in tendon healing.
Cite This Article
APA
Yuda Y, Kasashima Y, Kuwano A, Sato K, Hattori S, Arai K.
(2012).
Active hyaluronidase 2 expression in the granulation tissue formed in the healing process of equine superficial digital flexor tendonitis.
J Vet Med Sci, 75(2), 219-223.
https://doi.org/10.1292/jvms.12-0318 Publication
Researcher Affiliations
- Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Tokyo, Japan.
MeSH Terms
- Animals
- Forelimb
- Gene Expression Regulation, Enzymologic / physiology
- Granulation Tissue / enzymology
- Granulation Tissue / metabolism
- Horse Diseases / enzymology
- Horse Diseases / genetics
- Horse Diseases / metabolism
- Horses
- Hyaluronoglucosaminidase / classification
- Hyaluronoglucosaminidase / genetics
- Hyaluronoglucosaminidase / metabolism
- Male
- Tendinopathy / enzymology
- Tendinopathy / genetics
- Tendinopathy / metabolism
- Tendinopathy / veterinary
- Up-Regulation
Citations
This article has been cited 2 times.- Chan DD, Guilak F, Sah RL, Calve S. Mechanobiology of Hyaluronan: Connecting Biomechanics and Bioactivity in Musculoskeletal Tissues. Annu Rev Biomed Eng 2024 Jul;26(1):25-47.
- Kwon H, Park HS, Shim JY, Lee KW, Choi SJ, Choi GY. Randomized, Double-Blind, Placebo-Controlled Trial on the Efficacy of Hyaluronidase in Preventing Perineal Trauma in Nulliparous Women. Yonsei Med J 2020 Jan;61(1):79-84.
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