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Theriogenology2019; 140; 180-187; doi: 10.1016/j.theriogenology.2019.08.032

Activities of antioxidant seminal plasma enzymes (SOD, CAT, GPX and GSR) are higher in jackasses than in stallions and are correlated with sperm motility in jackasses.

Abstract: This study compared the activities of four antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPX; and glutathione reductase, GSR) in the seminal plasma of stallions and jackasses. Eighteen stallion ejaculates and 24 jack ejaculates were collected through an artificial vagina. Seminal plasma was obtained by several centrifugations at 3000×g and 4 °C for 10 min, and activities of SOD, CAT, GPX and GSR were subsequently determined. We also evaluated whether the collecting season had any influence on the activities of these four enzymes in both stallions and jackasses. Antioxidant capacity of seminal plasma was significantly higher in jackasses than in stallions (mean ± SEM, SOD: 1707.7 ± 195.9 U/mL vs. 231.9 ± 29.6 U/mL; CAT: 9094.7 ± 1292.9 U/L vs.1682.7 ± 525.9 U/L; GPX 845.4 ± 106.0 U/L vs. 469.7 ± 60.3 U/L; GSR: 50.3 ± 5.1 U/L vs. 20.7 ± 4.6 U/L). Furthermore, whereas season had no effect on the activity of these four enzymes in stallions, the activities of CAT and GPX in jack seminal plasma were significantly higher in the summer than in the other seasons. In addition, the activities of SOD and CAT were found to be significantly correlated with the percentages of progressively motile spermatozoa, and with the percentages of linearity and straightness, respectively, in jackasses. In contrast, the activities of these four enzymes were not correlated with sperm quality parameters in stallions. Finally, while SOD, CAT, and GPX activities but not those of GSR were correlated in jackasses, the activities of all four enzymes were correlated each other in stallions. We can thus conclude that the activities of SOD, CAT, GPX and GSR differ between the seminal plasma of stallions and donkeys, and vary between seasons in jackasses.
Copyright © 2019 Elsevier Inc. All rights reserved.
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Publication Date: 2019-08-31 PubMed ID: 31479834DOI: 10.1016/j.theriogenology.2019.08.032Google Scholar: Lookup
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Summary

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The research article compares the activities of four antioxidant enzymes in stallion and jackass seminal plasma, concluding that they are higher in jackasses and may play a role in sperm motility.

Methodology

  • The seminal samples were collected from 18 stallions and 24 jackasses, using an artificial vagina technique.
  • The collected seminal plasma was subjected to several round of centrifugations at a specific frequency and temperature to isolate the seminal plasma.
  • The levels of four crucial antioxidant enzymes — superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GSR) — were then determined in the plasma.

Observations

  • The antioxidant capacity, demonstrated by the activities of these antioxidant enzymes, were significantly higher in jackasses compared to stallions.
  • The season of collection had a noticeable influence on the activities of the enzymes in jackasses, particularly CAT and GPX, with higher values observed in the summer. On the other hand, the season did not impact the activities of the enzymes in stallions.

Findings Related to Sperm Quality

  • The activities of SOD and CAT in jackasses were correlated to sperm quality parameters such as the progressive motion of spermatozoa.
  • In contrast, the activities of these enzymes in stallions showed no correlation with sperm quality parameters.
  • The activities of SOD, CAT, and GPX in jackasses were correlated with each other. But all four enzymes showed correlated activities in stallions.

Conclusion

  • The research concluded that the activities of the four antioxidant enzymes — SOD, CAT, GPX, and GSR — differ between the seminal plasma of stallions and donkeys, and vary with seasons in jackasses.
  • These enzymes may play a crucial role in sperm quality and motility, particularly in jackasses.

Cite This Article

APA
Papas M, Arroyo L, Bassols A, Catalán J, Bonilla-Correal S, Gacem S, Yeste M, Miró J. (2019). Activities of antioxidant seminal plasma enzymes (SOD, CAT, GPX and GSR) are higher in jackasses than in stallions and are correlated with sperm motility in jackasses. Theriogenology, 140, 180-187. https://doi.org/10.1016/j.theriogenology.2019.08.032

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 140
Pages: 180-187
PII: S0093-691X(19)30378-4

Researcher Affiliations

Papas, Marion
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Universitat Autònoma Barcelona, Spain.
Arroyo, Laura
  • Department of Biochemistry and Molecular Biology, Faculty of Veterinary Sciences, Universitat Autònoma de Barcelona, Spain.
Bassols, Anna
  • Department of Biochemistry and Molecular Biology, Faculty of Veterinary Sciences, Universitat Autònoma de Barcelona, Spain.
Catalán, Jaime
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Universitat Autònoma Barcelona, Spain.
Bonilla-Correal, Sebastián
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Universitat Autònoma Barcelona, Spain.
Gacem, Sabrina
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Universitat Autònoma Barcelona, Spain.
Yeste, Marc
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, Girona, Spain. Electronic address: marc.yeste@udg.edu.
Miró, Jordi
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Universitat Autònoma Barcelona, Spain. Electronic address: jordi.miro@uab.cat.

MeSH Terms

  • Animals
  • Catalase / metabolism
  • Equidae
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Horses
  • Male
  • Semen / enzymology
  • Sperm Motility
  • Superoxide Dismutase / metabolism

Citations

This article has been cited 29 times.
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