Activity of coagulation and fibrinolysis parameters in animals.
Abstract: Using diagnostics for the determination of clotting factors and fibrinolytic parameters in human plasma, samples from rat, guinea pig, rabbit, cat, dog, sheep, cattle, horse, pig, and monkey were analysed. The human system was employed even for standard curves and controls. Results obtained in this way are relative values in relation to pooled fresh human plasma of healthy donors which is defined to contain 100% of the norm or 1 unit of each factor per 1 ml. Under these conditions, marked differences between the human clotting system and those of different animal species appear. Thus, rabbits have an about 50 times higher activity of factor V than humans and guinea pigs only 6% of the factor VII activity of human plasma. Plasma levels of fibrinogen, alpha 2-antiplasmin and antithrombin III in plasma samples of the investigated animal species are in the range of human plasma. Differences in the plasma level of single factors as compared to human plasma are reflected by clotting times of the screening tests. For the determination of plasminogen, streptokinase was used as activator of the fibrinolytic system. Hence, the results obtained by this method merely reflect the activatability of the animal plasminogen in comparison to the human system, however, do not allow statements concerning the real plasminogen content of the plasma sample from the animal species. For pharmacological investigations a proper selection of the animal species is important to prevent wrong conclusions.
Publication Date: 1994-06-01 PubMed ID: 8053983
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- Journal Article
Summary
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This research article examines the clotting factors and fibrinolytic parameters in various animal species including rat, guinea pig, rabbit, cat, dog, sheep, cattle, horse, pig, and monkey, in comparison to those parameters in human plasma.
Objective of Research
- The main objective of the research was to study the activity of coagulation and fibrinolysis parameters in different animal species and compare them with the parameters in human plasma. This was done with the use of diagnostic tools for clotting factors and fibrinolytic parameters.
Methodology
- The researchers analyzed plasma samples from different animal species by using the human system as a standard for curves and controls.
- The results obtained were relative values in relation to pooled fresh human plasma from healthy donors. This plasma is defined as containing 100% of the norm or 1 unit of each factor per 1 ml.
- For determining plasminogen, streptokinase was used as an activator of the fibrinolytic system.
Results
- Significant differences were found between the human clotting system and those of different animal species. For instance, rabbits have an activity of factor V that is approximately 50 times higher than in humans, while guinea pigs have only 6% of the factor VII activity of human plasma.
- The plasma levels of fibrinogen, alpha 2-antiplasmin, and antithrombin III in the plasma samples of the investigated animal species were found to be in the range of human plasma.
- Differences in the plasma level of single factors compared to human plasma correlated with clotting times of the screening tests.
Conclusions
- The research indicates that the results obtained by using streptokinase merely reflect the ability of the animal plasminogen to be activated in comparison to the human system. However, these results do not allow for any statements to be made about the real plasminogen content of the plasma sample from the animal species.
- The researchers concluded that the proper selection of animal species is important for pharmacological investigations to prevent inaccurate conclusions.
Cite This Article
APA
Karges HE, Funk KA, Ronneberger H.
(1994).
Activity of coagulation and fibrinolysis parameters in animals.
Arzneimittelforschung, 44(6), 793-797.
Publication
Researcher Affiliations
- Research Laboratories of Behringwerke AG, Marburg/Lahn, Fed. Rep. of Germany.
MeSH Terms
- Animals
- Blood Coagulation / physiology
- Blood Coagulation Factors / metabolism
- Cats
- Cattle
- Dogs
- Female
- Fibrinolysis / physiology
- Guinea Pigs
- Horses
- Humans
- In Vitro Techniques
- Macaca fascicularis
- Male
- Rabbits
- Rats
- Rats, Wistar
- Reference Standards
- Reference Values
- Sheep
- Species Specificity
- Swine
Citations
This article has been cited 17 times.- Liang Y, Tarandovskiy I, Surov SS, Ovanesov MV. Comparative Thrombin Generation in Animal Plasma: Sensitivity to Human Factor XIa and Tissue Factor. Int J Mol Sci 2023 Aug 18;24(16).
- Bowyer AE, Gosselin RC. Factor VIII and Factor IX Activity Measurements for Hemophilia Diagnosis and Related Treatments. Semin Thromb Hemost 2023 Sep;49(6):609-620.
- Guillen K, Comby PO, Salsac AV, Falvo N, Lenfant M, Oudot A, Sikner H, Dencausse A, Laveissiere E, Aho-Glele SL, Loffroy R. X-ray Microtomography to Assess Determinants of In Vivo N-Butyl Cyanoacrylate Glubran(®)2 Polymerization: A Rabbit-Model Study. Biomedicines 2022 Oct 19;10(10).
- Golub VM, Reddy DS. Post-Traumatic Epilepsy and Comorbidities: Advanced Models, Molecular Mechanisms, Biomarkers, and Novel Therapeutic Interventions. Pharmacol Rev 2022 Apr;74(2):387-438.
- Kostromina MA, Tukhovskaya EA, Shaykhutdinova ER, Slashcheva GA, Ismailova AM, Palikov VA, Palikova YA, Dyachenko IA, Kravchenko IN, Sadovnikova ES, Novikova NI, Perepechenova NA, Zayats EA, Abramchik YA, Lykoshin DD, Mamaev AN, Grigorieva EV, Momot AP, Murashev AN, Esipov RS. Screening of the Promising Direct Thrombin Inhibitors from Haematophagous Organisms. Part I: Recombinant Analogues and Their Antithrombotic Activity In Vitro. Biomedicines 2021 Dec 22;10(1).
- Wang L, Jiao L, Pang S, Yan P, Wang X, Qiu T. The Development of Design and Manufacture Techniques for Bioresorbable Coronary Artery Stents. Micromachines (Basel) 2021 Aug 20;12(8).
- Reed CR, Bonadonna D, Everitt J, Robinson V, Otto J, Tracy ET. Coagulopathy Characterized by Rotational Thromboelastometry in a Porcine Pediatric ECMO Model. J Extra Corpor Technol 2020 Sep;52(3):203-211.
- Tarandovskiy ID, Shin HKH, Baek JH, Karnaukhova E, Buehler PW. Interspecies comparison of simultaneous thrombin and plasmin generation. Sci Rep 2020 Mar 3;10(1):3885.
- Weber B, Lackner I, Haffner-Luntzer M, Palmer A, Pressmar J, Scharffetter-Kochanek K, Knöll B, Schrezenemeier H, Relja B, Kalbitz M. Modeling trauma in rats: similarities to humans and potential pitfalls to consider. J Transl Med 2019 Sep 5;17(1):305.
- Larsson M, Forsman P, Hedenqvist P, Östlund A, Hultman J, Wikman A, Riddez L, Frenckner B, Bottai M, Wahlgren CM. Extracorporeal membrane oxygenation improves coagulopathy in an experimental traumatic hemorrhagic model. Eur J Trauma Emerg Surg 2017 Oct;43(5):701-709.
- Roos ST, Yu FT, Kamp O, Chen X, Villanueva FS, Pacella JJ. Sonoreperfusion Therapy Kinetics in Whole Blood Using Ultrasound, Microbubbles and Tissue Plasminogen Activator. Ultrasound Med Biol 2016 Dec;42(12):3001-3009.
- Beyerle A, Solomon C, Dickneite G, Herzog E. Nonclinical analysis of the safety, pharmacodynamics, and pharmacokinetics of plasma-derived human FXIII concentrate in animals. Pharmacol Res Perspect 2016 Apr;4(2):e00227.
- Lemini C, Jaimez R, Figueroa A, Martinez-Mota L, Avila ME, Medina M. Ovariectomy differential influence on some hemostatic markers of mice and rats. Exp Anim 2015;64(1):81-9.
- Schürmann D, Herzog E, Raquet E, Nolte MW, May F, Müller-Cohrs J, Björkqvist J, Dickneite G, Pragst I. C1-esterase inhibitor treatment: preclinical safety aspects on the potential prothrombotic risk. Thromb Haemost 2014 Nov;112(5):960-71.
- Renz BW, Leitner K, Odermatt E, Worthley DL, Angele MK, Jauch KW, Lang RA. PVA gel as a potential adhesion barrier: a safety study in a large animal model of intestinal surgery. Langenbecks Arch Surg 2014 Mar;399(3):349-57.
- Gunerhan Y, Koksal N, Kayahan M, Eryavuz Y, Sekban H. Diagnostic value of plasminogen activity level in acute mesenteric ischemia. World J Gastroenterol 2008 Apr 28;14(16):2540-3.
- Kruse P, Hage E, Lasson A. Proteases and protease inhibitors in taurocholate-induced acute pancreatitis in rats. Int J Pancreatol 1999 Apr;25(2):113-21.
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