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Theriogenology2005; 63(5); 1354-1365; doi: 10.1016/j.theriogenology.2004.07.005

Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5 degrees C.

Abstract: Sperm cell membranes are susceptible to peroxidative damage by an excess of reactive oxygen species (ROS). Antioxidative defence systems consisting of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) physiologically control the balance between ROS production and neutralization. In the present study the hypothesis was tested that lipid peroxidation occurs during storage of semen at 5 degrees C and that semen extender has positive effects on the antioxidative potential of equine semen. The aim of the study was to determine the activity of GSH-Px, SOD and CAT and the concentration of thiobarbituric acid reactive substances (TBARS) as an indicator of lipid peroxidation in native semen and after addition of extender, cooling and storage. Semen was collected from fertile Shetland stallions. In experiment 1, activity of antioxidative enzymes was determined immediately after semen collection and after 24 h storage at 5 degrees C. Enzyme activities were measured in native semen, semen diluted with semen extender, spermatozoa resuspended after centrifugation in extender and 0.9% NaCl as well as in undiluted and extender-diluted seminal plasma. In experiment 2, TBARS concentrations were analysed during storage of semen at 5 degrees C for 24 h. Semen storage for 24 h at 5 degrees C did not change activity of the examined enzymes. Antioxidative activity was significantly higher in extended than in native semen as well as in extended plasma than in undiluted plasma. In conclusion, the addition of semen extender increases the antioxidative activity in seminal plasma of stallions. Basal antioxidative activity in native semen as well as increased activity in extended semen are maintained over 24 h storage at 5 degrees C. TBARS content did not increase during semen storage. In conclusion, lipid peroxidation does not increase substantially during semen storage. The enzymatic antioxidative activity in semen apparently prevents ROS formation and is further increased by addition of semen extender.
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Publication Date: 2005-02-24 PubMed ID: 15725443DOI: 10.1016/j.theriogenology.2004.07.005Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effects of storing stallion semen at 5 degrees Celsius on the activities of certain antioxidative enzymes and lipid peroxidation levels. It finds that the cooling process doesn’t alter the functionality of the enzymes, and that adding a semen extender increases the antioxidative activity without leading to significant lipid peroxidation during storage.

Detailed Explanation of the Research Paper

Objective and Hypothesis

  • The research was aimed at determining the effects of storage at 5 degrees Celsius on equine semen, particularly on the activities of antioxidative enzymes namely, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT).
  • The researchers also wanted to establish whether lipid peroxidation, a process that can inflict damage on sperm cells, occurs during storage and if a semen extender can enhance the semen’s antioxidative capacity.

Methods

  • Semen was collected from fertile Shetland stallions and subject to different conditions like immediate measurement after collection, dilution with semen extender, resuspension in extender and 0.9% NaCl after centrifugation, and storage at 5 degrees Celsius for 24 hours.
  • The researchers measured the activities of the three antioxidative enzymes and the concentration of thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation.

Results

  • It was found that the storage for 24 hours at 5 degrees Celsius did not alter the activity of the examined enzymes.
  • It was also observed that the antioxidative activity was significantly higher in semen extended than in the native semen as well as in extended plasma than in undiluted plasma, indicating the positive effects of a semen extender.
  • Interestingly, the TBARS content did not increase during storage, suggesting that lipid peroxidation does not increase significantly during semen storage.

In summary, the study found that storage of stallion semen at 5 degrees Celsius maintains the antioxidative enzyme activities without leading to substantial lipid peroxidation. Moreover, the use of a semen extender further increases the antioxidative activity in stallion semen.

Cite This Article

APA
Kankofer M, Kolm G, Aurich J, Aurich C. (2005). Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5 degrees C. Theriogenology, 63(5), 1354-1365. https://doi.org/10.1016/j.theriogenology.2004.07.005

Publication

Theriogenology
ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 63
Issue: 5
Pages: 1354-1365

Researcher Affiliations

Kankofer, Marta
  • Department of Animal Breeding and Reproduction, University of Veterinary Sciences, 1210 Vienna, Veterinärplatz 1, Austria. Marta.Kankofer@vu-wien.ac.at
Kolm, Gabriela
    Aurich, Jörg
      Aurich, Christine

        MeSH Terms

        • Animals
        • Antioxidants / metabolism
        • Catalase / metabolism
        • Centrifugation
        • Glutathione Peroxidase / metabolism
        • Horses / metabolism
        • Lipid Peroxidation
        • Male
        • Reactive Oxygen Species / metabolism
        • Semen / chemistry
        • Semen / metabolism
        • Semen Preservation / methods
        • Semen Preservation / veterinary
        • Solutions
        • Superoxide Dismutase / metabolism
        • Thiobarbituric Acid Reactive Substances / analysis

        Citations

        This article has been cited 13 times.
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